Visualizing the Effect of Process Pause on Virus Entrapment During Constant Flux Virus Filtration.

Wenbo Xu, Xianghong Qian, Hironobu Shirataki, Daniel Straus, Sumith Ranil Wickramasinghe

Abstract read

In one paragraph

Article in Membranes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Wenbo XuDepartment of Biomedical Engineering, University of Arkansas, 1475 West Cato Springs Road, Fayetteville, AR 72701, USA.ORCID 0009-0008-9999-7117

Xianghong QianDepartment of Biomedical Engineering, University of Arkansas, 1475 West Cato Springs Road, Fayetteville, AR 72701, USA.ORCID 0000-0001-5619-9782

Hironobu ShiratakiBioprocess Division, Asahi Kasei Life Science Corporation, Hibiya Mitsui Tower, 1-1-2 Yurakucho, Chiyoda-ku, Tokyo 100-0006, Japan.

Daniel StrausPurification Research and Development, Asahi Kasei Bioprocess America, Inc., 1855 Elmdale Ave, Glenview, IL 60026, USA.

Sumith Ranil WickramasingheRalph E. Martin Department of Chemical Engineering, University of Arkansas, 1475 West Cato Springs Road, Fayetteville, AR 72701, USA.ORCID 0000-0001-7436-0517

Funding

Asahi Kasei Life Sciences Corporation, Not Applicable

6 · The paper itself

Abstract

Virus filtration is an essential unit operation used to validate clearance of adventitious virus during the manufacture of biopharmaceutical products such as monoclonal antibodies. Obtaining at least a 10,000-fold reduction in virus particles in the permeate is challenging as monoclonal antibodies are about half the size of the virus particles. Minute virus of mice, FDA-recommended model adventitious virus, was labeled with a fluorescent dye. Laser scanning confocal microscopy was used to determine the location of virus entrapment within the virus filtration membrane. Three different hollow fiber membranes made of regenerated cellulose and polyvinylidene fluoride were tested. Feed streams consisted of MVM spiked in buffer and MVM spiked in 5 g L

Indexed as

aggregationfluxhollow fiberlaser scanning confocal microscopylog removal of virusminute virus of micemonoclonal antibodypolymeric membraneprocess interruptionvirus clearance

Identifiers

PMID41590559

DOI10.3390/membranes16010006

PMCPMC12844474

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.