Validation and Improvement of a Rapid, CRISPR-Cas-Free RPA-PCRD Strip Assay for On-Site Genomic Surveillance and Quarantine of Wheat Blast.

Dipali Rani Gupta, Shamfin Hossain Kasfy, Julfikar Ali, Farin Tasnova Hia, M Nazmul Hoque, Mahfuz Rahman, Tofazzal Islam

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In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Dipali Rani GuptaInstitute of Biotechnology and Genetic Engineering (IBGE), Gazipur Agricultural University, Gazipur 1706, Bangladesh.

Shamfin Hossain KasfyInstitute of Biotechnology and Genetic Engineering (IBGE), Gazipur Agricultural University, Gazipur 1706, Bangladesh.

Julfikar AliInstitute of Biotechnology and Genetic Engineering (IBGE), Gazipur Agricultural University, Gazipur 1706, Bangladesh.ORCID 0009-0007-7383-8532

Farin Tasnova HiaInstitute of Biotechnology and Genetic Engineering (IBGE), Gazipur Agricultural University, Gazipur 1706, Bangladesh.ORCID 0009-0001-9481-7761

M Nazmul HoqueMolecular Biology and Bioinformatics Laboratory, Department of Gynaecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur 1706, Bangladesh.ORCID 0000-0002-4861-0030

Mahfuz RahmanExtension Service, West Virginia University, Morgantown, WV 26506, USA.ORCID 0000-0002-7049-5796

Tofazzal IslamInstitute of Biotechnology and Genetic Engineering (IBGE), Gazipur Agricultural University, Gazipur 1706, Bangladesh.ORCID 0000-0002-7613-0261

Funding

Bill and Melinda Gates Foundation and the Foreign, Commonwealth & Development Office (FCDO), UK Grant Code: V0156.01

6 · The paper itself

Abstract

As an emerging threat to global food security, wheat blast necessitates the development of a rapid and field-deployable detection system to facilitate early diagnosis, enable effective management, and prevent its further spread to new regions. In this study, we aimed to validate and improve a Recombinase Polymerase Amplification coupled with PCRD lateral flow detection (RPA-PCRD strip assay) kit for the rapid and specific identification of

Indexed as

early warning advisory systemfield diagnosisIPMlateral flow assayMagnaporthe oryzae Triticumpoint-of-care detection

Identifiers

PMID41590485

DOI10.3390/jof12010073

PMCPMC12842714

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.