Evidence map›Paper›PMID 41590303›Full record

ArticleBiosensors2026

A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing.

Baihui Zhang, Xiao Li, Mengjie Huang, Maojie Jiang, Leilei Du, Peng Yin, Xuan Fang, Xiangyu Jiang, Feihu Qi, Yanna Lin and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Baihui ZhangShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.
Xiao LiMedical Enzyme Engineering Center, CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215613, China.
Mengjie HuangShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.
Maojie JiangShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.
Leilei DuWeihai Huakang Medical Laboratory Co., Ltd., Weihai 264210, China.
Peng YinShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.ORCID 0000-0001-7152-9882
Xuan FangShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.
Xiangyu JiangShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.ORCID 0009-0007-2842-6256
Feihu QiSuzhou Ker Life Technology Co., Ltd., Suzhou 215163, China.
Yanna LinShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.
Fuqiang MaShandong Lab of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264210, China.

Funding

China Postdoctoral Science Foundation 2022M722337Chinese Academy of Sciences YJKYYQ20210032Excellent Postdoctoral Program in Jiangsu Province 2022ZB874, 2023ZB046National Natural Science Foundation of China 32471321Natural Science Foundation of Shandong Province, China ZR2023QB240, ZR2023QC257Projects of the National Key R&D Program of China 2025YFF1207801, 2022YFF0710101Shandong Provincial Laboratory Project SYS202209Youth Innovation Promotion Association Fellowship Program, CAS 2022327
6 · The paper itself

Abstract

Point-of-care testing (POCT) has emerged as a vital diagnostic approach in emergency medicine, primary care, and resource-limited environments because of its convenience, affordability, and capacity to provide immediate results. Here, we present a multifunctional portable nucleic acid detection platform integrating reverse transcription polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) within a unified microfluidic device. The system leverages Tesla-valve-based passive flow control to enhance reaction efficiency and operational simplicity. A four-channel optical detection unit allows for multiplex fluorescence quantification (CY5, FAM, VIC, ROX) and has high sensitivity and reproducibility for RT-LAMP. The compact design reduces the overall size by approximately 90% compared with conventional qPCR instruments. For RT-PCR, the system achieves a detection limit of 2.0 copies μL

Indexed as

Lab-On-A-Chip DevicesNucleic Acid Amplification TechniquesNucleic AcidsPoint-of-Care TestingHumansLimit of DetectionMolecular Diagnostic TechniquesPoint-of-Care SystemsRapid Diagnostic TestsReal-Time Polymerase Chain ReactionNucleic AcidsPOCTRT-LAMPRT-qPCRTesla valvesTV-qPCR

Identifiers

PMID41590303
PMCPMC12839313

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.