Evidence map›Paper›PMID 41588633›Full record

ArticleJournal of peptide science : an official publication of the European Peptide Society2026

An Effective Method for Isolating Ergothioneine Analogues for Use in Peptide Synthesis: 2-Thiohistidine and Fmoc-2-Thiohistidine.

Quinn A Bennett, Matthew Chien, Erik L Ruggles, Robert J Hondal, Emma J Barrett

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In one paragraph

Article in Journal of peptide science : an official publication of the European Peptide Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Quinn A BennettDepartment of Chemistry, University of Vermont, Burlington, Vermont, USA.
Matthew ChienDepartment of Chemistry, University of Vermont, Burlington, Vermont, USA.
Erik L RugglesDepartment of Chemistry, University of Vermont, Burlington, Vermont, USA.
Robert J HondalDepartment of Biochemistry, University of Vermont, Burlington, Vermont, USA.
Emma J BarrettDepartment of Chemistry and Biochemistry, Norwich University, Northfield, Vermont, USA.

Funding

Vermont INBRE Administrative Supplement 2024 AWD 118P20GM103449 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI Julie Dragon · 2012 to 2026
$58.4M
NIGMS NIH HHS P20 GM103449NIGMS NIH HHS P20GM103449
6 · The paper itself

Abstract

Here, we report on an improved synthesis of the novel amino acid 2-thiohistidine and its Fmoc-derivative through the addition of sodium chloride to the isolation steps of each synthetic procedure. These compounds must be synthesized in either water or a mixture of water/organic solvent and have proven difficult to isolate by traditional methods. We serendipitously discovered that the addition of sodium chloride caused each compound to precipitate from the solution during the workup. Since 2-thiohistidine is the amino acid analogue of the natural product ergothioneine, we hypothesize that sodium cations mimic the binding of other biologically relevant metal cations to the thioimidazole moiety. Ergothioneine is known to bind Cu

Indexed as

ErgothioneineFluorenesHistidinePeptidesErgothioneineFluorenesHistidinePeptides

Identifiers

PMID41588633
PMCPMC13213726

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.