Evidence map›Paper›PMID 41588333›Full record

ArticleBMC genomics2026

Integrated analysis of lncRNA-miRNA-mRNA networks reveals stage-specific molecular regulation of oogenesis in Chinese alligator (Alligator sinensis).

Peng Liu, Ruiyun Liu, Fuyong You, Yuan Zhang, Shaofan Li, Xinxin Zhang, Xiaobing Wu, Haitao Nie

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peng LiuAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Ruiyun LiuAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Fuyong YouAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Yuan ZhangAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Shaofan LiAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Xinxin ZhangAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China.
Xiaobing WuAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China. wuxb@ahnu.edu.cn.
Haitao NieAnhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, China. niehaitao@ahnu.edu.cn.

Funding

National Natural Science Foundation of China 32370542National Natural Science Foundation of China 32470521
6 · The paper itself

Abstract

backgroundReptiles, as the earliest vertebrate group to fully adapt to terrestrial environments, have reproductive strategies that hold critical significance for understanding biological evolution and ecological adaptation. However, the mechanisms of oogenesis in reptiles, particularly in the endangered Chinese alligator (Alligator sinensis), remain poorly understood.

resultsHere we integrated transcriptomic and non-coding RNA analyses to elucidate stage-specific lncRNA/miRNA-mRNA regulatory networks during oogenesis. Gonadal tissue samples were collected at three critical developmental stages: 1-day post-hatching (AH), 15-day post-hatching (BH), and 90-day post-hatching (CH). RNA sequencing revealed 568 upregulated and 222 downregulated genes during the early proliferation phase (from AH period to BH period), and 667 upregulated and 241 downregulated genes during the maturation phase (from BH period to CH period). Additionally, we identified 1,194 lncRNAs and 1,808 miRNAs dynamically expressed across these stages. During early proliferation, cell cycle genes (CCN3 - CCN Family Member 3, CDC45 - Cell Division Cycle 45) and signaling pathways (WNT - Wingless-Type MMTV Integration Site Family, FGF - Fibroblast Growth Factor) dominated, while metabolic genes (ALDOB-Aldolase B, Fructose-Bisphosphate, FABP4 - Fatty Acid Binding Protein 4) were suppressed. In the maturation phase, meiosis-related genes (STAG3 - Stromal Antigen 3, SPO11- Sporulation Protein 11 Homolog) and germ cell-specific factors (DAZL - Deleted in Azoospermia-Like, NOBOX - Newborn Ovary Homeobox) were upregulated, accompanied by miRNA-mediated suppression of non-essential functions. This study provides the first comprehensive roadmap of non-coding RNA-mediated oogenesis in reptiles, offering insights for conservation strategies in endangered species.

Indexed as

Alligators and CrocodilesGene Regulatory NetworksMicroRNAsOogenesisRNA, Long NoncodingRNA, MessengerAnimalsFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalMicroRNAsRNA, Long NoncodingRNA, MessengerCell cycleChinese alligatorLncRNAMeiosisMiRNAOogenesisRegulatory network

Identifiers

PMID41588333
PMCPMC12918031

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.