Development of a new biological dressing: the modified cross-linking of Chitosan derived from cuttlefish (Sepia pharaonis) by-products as an effective agent for promoting cell migration.

Ping-Hsiu Huang, Yu-Wei Chen, Jing-Huei Zeng, Bo-Heng Li, Ya-Ting Chen, Shu-Ling Hsieh, Ming-Kuei Shih, Chih-Yao Hou

Abstract read

In one paragraph

Article in Bioresources and bioprocessing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Ping-Hsiu Huang *Department of Food Science, College of Life Science, National Chiayi University, No.300 Syuefu Rd, Chiayi City, 600355, Taiwan.

Yu-Wei Chen *Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, 40227, Taiwan.

Jing-Huei ZengDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung. University of Science and Technology, Kaohsiung, 81157, Taiwan.

Bo-Heng LiDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung. University of Science and Technology, Kaohsiung, 81157, Taiwan.

Ya-Ting ChenDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung. University of Science and Technology, Kaohsiung, 81157, Taiwan.

Shu-Ling HsiehDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung. University of Science and Technology, Kaohsiung, 81157, Taiwan.

Ming-Kuei ShihGraduate Institute of Food Culture and Innovation, National Kaohsiung University of Hospitality and Tourism, Kaohsiung, 812301, Taiwan. mkshih@mail.nkuht.edu.tw.

Chih-Yao HouDepartment of Seafood Science, College of Hydrosphere, National Kaohsiung. University of Science and Technology, Kaohsiung, 81157, Taiwan. chihyaohou@gmail.com.ORCID http://orcid.org/0000-0002-8007-6077

Funding

National Science and Technology Council funded this research, Republic of China 111-2221-E-328-001-MY3; 112-2221-E-992-002-MY3 and 113-2622-E-992-014 -

6 · The paper itself

Abstract

This study aimed to extract chitosan (CS) from cuttlefish (Sepia pharaonis) bones (CB) and then chemically modify it to produce carboxymethyl chitosan (CMC). Marine cuttlefish skin collagen peptide (MCP) was then cross-linked with CMC to form a novel CMC-MCP complex. The physicochemical properties and biological effects of CS, CMC, MCP, and CMC-MCP were evaluated using human keratinocyte (HaCaT) cell lines. All materials showed cytotoxicity at high concentrations (100-1600 µg/mL), negatively affecting cell viability. At a lower concentration of 50 µg/mL, the materials were used to assess cell migration. Among them, the CMC-MCP complex significantly promoted cell migration. Additionally, CMC-MCP treatment led to increased expression levels of matrix metalloproteinases (MMP-2 and 9) and tissue inhibitors of metalloproteinases (TIMP-1 and 2), which are key regulators in the wound healing process. These findings suggest that the CMC-MCP complex has potential as an economical, safe, and effective biological dressing for promoting wound healing. Further studies are recommended to explore its interaction with other healing-related factors, such as nutrients and growth factors, to better understand its influence on various stages of tissue repair.

Indexed as

BiomaterialGene expressionHaCaT cell linesMatrix metalloproteinasesWound healing

Identifiers

PMID41588206

DOI10.1186/s40643-025-01002-3

PMCPMC12835482

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