Evidence map›Paper›PMID 41588166›Full record

ArticleOdontology2026

Bulk RNA sequence analysis of the articular disc-retrodiscal tissue complex in a TMJ-OA rat model with anterior disc displacement.

Daiki Kita, Naoto Hirose, Makoto Yanoshita, Shota Ito, Yuma Koizumi, Sayuri Nishiyama, Eri Tsuboi, Naoki Kubo, Risako Oshiro, Marino Yoshida and 2 more

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Article in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Daiki KitaDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Naoto HiroseDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan. hirose@hiroshima-u.ac.jp.ORCID http://orcid.org/0000-0003-3354-6694
Makoto YanoshitaDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Shota ItoDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Yuma KoizumiDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Sayuri NishiyamaDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Eri TsuboiDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Naoki KuboDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Risako OshiroDepartment of Orthodontics, Hiroshima University Graduate School of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Marino YoshidaDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Yuki AsakawaDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.
Kotaro TanimotoDepartment of Orthodontics and Craniofacial Developmental Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8553, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveArticular disc displacement (ADD) is a major pathology of temporomandibular disorders that increases temporomandibular joint osteoarthritis (TMJ-OA) risk. However, its effect on gene expression in the retrodiscal tissue remains unexplored. This research elucidated the genetic alterations in the articular disc-retrodiscal tissue complex (ADRC) caused by ADD and investigated their association with TMJ-OA.

methodsADD was induced in the temporomandibular joints of rats, which were euthanised after 8 weeks post-op. RNA sequencing was performed on the ADRC (n = 3/group), and histological analysis was conducted on temporomandibular joints (n = 3/group).

resultsExpression analysis identified 103 significantly upregulated and 129 significantly downregulated genes in the ADRC of ADD rats. Differentially expressed genes analysis revealed a significant upregulation of growth differentiation factor-10 (log2FC = 1.16), suggesting its role in mandibular condyle deformity. Gene ontology analysis revealed significant upregulation of processes related to structural and nervous system development and significant downregulation of fat metabolism processes. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed a significant increase in inflammatory and osteoclast differentiation pathways, whilst adipocytokine signalling, linoleic acid metabolism pathways, and circadian rhythm were significantly downregulated.

conclusionThis is the first study to establish genetic changes with the ADRC in ADD and identify factors related to TMJ-OA pathologies.

Indexed as

Joint DislocationsOsteoarthritisSequence Analysis, RNATemporomandibular Joint DiscTemporomandibular Joint DisordersAnimalsDisease Models, AnimalMaleRatsRats, Sprague-DawleyArticular disc displacementBulk RNA-seq analysisTemporomandibular disordersTemporomandibular joint osteoarthritis

Identifiers

PMID41588166

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.