Evidence map›Paper›PMID 41587643›Full record

ArticleThe Journal of nutritional biochemistry2026

A metabolic basis for motor deficits in mice lacking BCKDK.

Lingyi Zhu, Isha Kinjawadekar, Caleb Prempeh, Inna A Nikonorova, Emily T Mirek, Esther M Lopez, William O Jonsson, Jordan L Levy, Lin Wang, Xiaoxuan Li and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutritional biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Lingyi ZhuDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Isha KinjawadekarDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Caleb PrempehDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Inna A NikonorovaDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Emily T MirekDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Esther M LopezDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
William O JonssonDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Jordan L LevyDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Lin WangDepartment of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
Xiaoxuan LiDepartment of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
Yeva ShamailovaDepartment of Animal Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Timothy J StanekDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Elizabeth M SnyderDepartment of Animal Sciences, Rutgers University, New Brunswick, New Jersey, USA.
Piyawan BunpoDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Robert A HarrisDepartment of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Shawn M DavidsonDepartment of Molecular Biology, Princeton University, Princeton, New Jersey, USA; Division of Pulmonary and Critical Care, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA.
Ronald C WekDepartment of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Tracy G AnthonyDepartment of Nutritional Sciences, Rutgers University, New Brunswick, New Jersey, USA. Electronic address: tracy.anthony@rutgers.edu.

Funding

Homeostatic Responses to Amino Acid InsufficiencyR01DK109714 · NIDDK · RUTGERS, THE STATE UNIV OF N.J. · PI ANTHONY, TRACY G., WEK, RONALD C · 2016 to 2024
$5.4M
NIDDK NIH HHS R01 DK109714
6 · The paper itself

Abstract

Branched-chain α-amino acids (BCAAs) support protein synthesis and their oxidation is restrained by branched-chain α-keto acid dehydrogenase kinase (BCKDK). We previously observed that in the brains of Bckdk knockout (KO) mice, BCAAs fall while glutamate is preserved and other amino acids rise. We asked why this profile emerges and how it affects skeletal muscle versus brain during nutrient stress. Motor behavior, protein synthesis and nutrient signaling were compared in the skeletal muscle and brains of wildtype (WT) and Bckdk KO male mice. In addition, nitrogen delivery into brain from BCAAs was assessed using stable isotope tracing and mass spectrometry imaging. Bckdk KO showed normal grip strength but poor beam traversal and reduced wheel running during protein restriction. In skeletal muscle, leucine or protein-feeding stimulated and fasting suppressed mechanistic target of rapamycin complex 1 (mTORC1) signaling in both genotypes. Fasting reduced muscle protein synthesis in both strains without activating the integrated-stress response (ISR). In contrast, Bckdk KO brains exhibited ISR activation during fasting, and up-regulation of Atf4 and its target genes, including Slc7a5 mRNA. Tracer studies revealed lower serum [

Indexed as

Motor ActivityAmino Acids, Branched-ChainAnimalsBrainMaleMechanistic Target of Rapamycin Complex 1MiceMice, KnockoutMuscle, SkeletalProtein KinasesSignal Transduction(3-methyl-2-oxobutanoate dehydrogenase (lipoamide)) kinaseAmino Acids, Branched-ChainMechanistic Target of Rapamycin Complex 1Protein KinasesBCAAsBCKDKGlutamateMALDI imagingNeuromuscular coordinationStable isotope tracing

Identifiers

PMID41587643
PMCPMC13361005

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.