Evidence map›Paper›PMID 41586020›Full record

ArticleOncology letters2026

Cytotoxic, apoptotic and genotoxic effects of thymoquinone-oxime derivative on gastric cancer cells: An

Kübra Bozali, Zeynep İnce, Nurhilal Kiziltoprak, Taygun Gülşen, Mehmet Köstek, Yasir Musa Kesgin, Muhammer Ergenç, Mine Dağgez, Eren Altun, Fatih Taşkesen and 4 more

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In one paragraph

Article in Oncology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Kübra BozaliDepartment of Medical Biochemistry, Faculty of Hamidiye Medicine, University of Health Sciences, Istanbul 34668, Türkiye.
Zeynep İnceDepartment of Medical Biochemistry, Faculty of Hamidiye Medicine, University of Health Sciences, Istanbul 34668, Türkiye.
Nurhilal KiziltoprakDepartment of General Surgery, University of Health Sciences, Sultan 2. Abdulhamid Han Training and Research Hospital, Istanbul 34668, Türkiye.
Taygun GülşenDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Mehmet KöstekDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Yasir Musa KesginDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Muhammer ErgençDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Mine DağgezDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Eren AltunDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Fatih TaşkesenDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Cebrail AkyüzDepartment of General Surgery, Haydarpasa Numune Health Application and Research Center, Istanbul 34668, Türkiye.
Oğuzhan SunamakDepartment of General Surgery, Haydarpasa Numune Health Application and Research Center, Istanbul 34668, Türkiye.
Mustafa DumanDepartment of Molecular Oncology, Hamidiye Institute of Health Sciences, University of Health Sciences, Istanbul 34668, Türkiye.
Eray Metin GülerDepartment of Medical Biochemistry, Faculty of Hamidiye Medicine, University of Health Sciences, Istanbul 34668, Türkiye.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastric cancer (GC) remains a notable global health concern, emphasizing the need for novel and effective therapeutic agents. The present study investigated the cytotoxic, genotoxic and apoptotic effects of thymoquinone-oxime (TQ-ox) on human gastric adenocarcinoma AGS cells and assessed its potential to induce DNA damage. Cytotoxicity was evaluated in AGS and normal human gastric epithelial cells (HGEpiCs) using a luminometric ATP assay. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were measured fluorometrically, whereas intracellular glutathione (GSH) content was determined via a luminometric GSH assay kit. DNA damage was quantified by a comet assay, and apoptosis was assessed by fluorescence microscopy with acridine orange/ethidium bromide (AO/EB) double staining. TQ-ox exhibited dose-dependent cytotoxicity in gastric cells, with AGS cells having a slightly higher sensitivity to TQ-ox treatment than HGEpiCs [half-maximal inhibitory concentration (IC

Indexed as

cytotoxicitygastric cancergenotoxicityoximethymoquinone

Identifiers

PMID41586020
PMCPMC12828300

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.