Evidence map›Paper›PMID 41582668›Full record

ArticleBiochemistry2026

Mapping RNA-Binding Proteins on the Ribosome by Tethered Micrococcal Nuclease.

Chia Yi Yao, Simpson Joseph

Abstract read
In one paragraph

Article in Biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Chia Yi YaoDepartment of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093-0314, United States.
Simpson JosephDepartment of Chemistry and Biochemistry, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093-0314, United States.ORCID 0000-0002-0552-8221

Funding

Mechanism of Protein Synthesis and Translational ControlR35GM141864 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JOSEPH, SIMPSON · 2021 to 2025
$2.0M
NIGMS NIH HHS R35 GM141864
6 · The paper itself

Abstract

RNA-binding proteins (RBPs) are essential regulators of posttranscriptional gene expression, influencing mRNA processing, translation, and stability. Defining their binding sites on RNA is key to understanding how they assemble into functional ribonucleoprotein (RNP) complexes, but existing footprinting and cross-linking approaches often yield low signal-to-noise, variable efficiency, or require highly purified complexes. To address these limitations, we developed Tethered Micrococcal Nuclease Mapping (TM-map), a sequencing-based strategy that determines the three-dimensional binding sites of RBPs on RNA in vitro. In TM-map, the RBP is fused to micrococcal nuclease (MNase), which upon Ca

Indexed as

Micrococcal NucleaseRibosomesRNA-Binding ProteinsAnimalsBinding SitesDrosophila ProteinsEscherichia coliDrosophila ProteinsMicrococcal NucleaseRNA-Binding Proteins

Identifiers

PMID41582668
PMCPMC13188847

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.