ArticleAnalytical and bioanalytical chemistry2026
Sensitive and selective detection of microRNA using hyperbranched rolling circle amplification based on symmetric dumbbell-type probe.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Examining the changes in microRNA expression in vivo enhances the understanding of their tumor-associated functions and facilitates clinical assays based on their roles as disease markers. However, due to the low abundance and high homology, their detection remains challenging. Herein, we developed an exponential amplification method by combining a symmetric dumbbell-type probe (SDTP) and toehold-initiated hyperbranched rolling circle amplification (HRCA) to obtain high sensitivity and selectivity. The toehold region of the dumbbell-type probe specifically binds to the target, triggering a toehold-mediated strand displacement reaction and thus activating SDTP into a circular structure. In the presence of phi29 DNA polymerase, primer P2, and dNTPs, the HRCA reaction proceeds continuously using the circular structure as a template, enabling exponential amplification of let-7a. When the concentration of let-7a ranges from 1.00 fmol·L
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