ArticleCommunications biology2026
Genome sequence assembly of the 5S rDNA loci informs haplotype specificity and evolution in the greater duckweed Spirodela polyrhiza.
Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Newly unveiled meiosis elucidates the unreduced gamete frequency and its impact on the evolution of the Lemna minor complex.Journal of experimental botany · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite the rapid expansion of information on eukaryotic genomes, data on ribosomal DNA (rDNA) loci encoding ribosomal RNAs, crucial for the biogenesis of ribosomes, are absent in almost all cases due to difficulties in assembling the long regions of tandemly repeated DNA units. Taking advantage of the uniquely low rDNA copy number in the aquatic plant Spirodela polyrhiza, we resolved the species' complete 5S rDNA architecture at a nucleotide level. A combination of in situ hybridization, extra-long DNA reads, and conventional DNA sequencing allowed the assembly of near-complete loci sequences of 40,878 bp, specific for one haplotype of chromosome ChrSp6, and of 110,911 bp specific for a haplotype of ChrSp13. The completely resolved 5S rDNA locus of ChrSp6 contains 40 copies of tandemly repeated gene units with an intergenic spacer (NTS) of 400 bp for one haplotype, and more than 60 highly homogenized gene copies for the second haplotype. The ChrSp13 locus contains 5S rDNA clusters with NTSs of 1,056 or 1,069 bp arranged in two sub-clusters. The G/C-rich 5S rDNA arrays in both loci are embedded in A/T-enriched chromosome regions. This work advances our understanding of the basic principles of rDNA organization and evolution of rRNA genes in plants by revealing the molecular architecture and evolutionary dynamics of 5S rDNA loci.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.