Evidence map›Paper›PMID 41581881›Full record

ArticleThe Journal of biological chemistry2026

Target validation uncouples mitochondrial translocator protein from 19-Atriol-mediated inhibition of steroidogenesis and identifies enzymatic targets.

Amy H Zhao, Prasanthi P Koganti, Mingxing Qian, Anthony Garcia, Patrick O'Day, Richard J Auchus, Douglas F Covey, Vimal Selvaraj

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Amy H ZhaoDepartment of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, USA.
Prasanthi P KogantiDepartment of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, USA.
Mingxing QianDepartment of Developmental Biology, School of Medicine, Washington University, St Louis, Missouri, USA.
Anthony GarciaDepartments of Pharmacology and Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
Patrick O'DayDepartments of Pharmacology and Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
Richard J AuchusDepartments of Pharmacology and Internal Medicine, University of Michigan, Ann Arbor, Michigan, USA.
Douglas F CoveyDepartment of Developmental Biology, School of Medicine, Washington University, St Louis, Missouri, USA; Taylor Family Institute for Innovative Psychiatric Research, School of Medicine, Washington University, St Louis, Missouri, USA.
Vimal SelvarajDepartment of Animal Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, USA. Electronic address: vs88@cornell.edu.

Funding

Activation of androgen biosynthesis and drug metabolism by cytochrome b5R01GM086596 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI RICHARD J. AUCHUS · 2009 to 2026
$4.8M
NIGMS NIH HHS R01 GM086596
6 · The paper itself

Abstract

The mitochondrial translocator protein (TSPO) was once proposed to mediate mitochondrial cholesterol import for steroid hormone biosynthesis, but genetic deletion studies in multiple models have refuted this role. Nevertheless, the idea that pharmacological ligands of TSPO can modulate steroid output continues to be invoked. One such compound, 19-Atriol (androst-5-ene-3β,17β,19-triol), was reported to inhibit progesterone synthesis via TSPO binding in MA-10 Leydig cells. To evaluate this proposed mechanism, we used CRISPR/Cas9-generated Tspo-deleted MA-10 cells to study 19-Atriol activity. We found that 19-Atriol inhibited Bt

Indexed as

AndrostenesEnzyme InhibitorsMitochondriaReceptors, GABASteroids3-Hydroxysteroid DehydrogenasesAnimalsCell LineLeydig CellsMaleMicePregnenoloneProgesterone3-beta-(2-(diethylamino)ethoxy)androst-5-en-17-one3-Hydroxysteroid DehydrogenasesAndrostenesBzrp protein, mouseEnzyme InhibitorsPregnenoloneProgesteroneReceptors, GABASteroidsadrenalcholesterolhormonemitochondriaovarysteroidsteroidogenesistestis

Identifiers

PMID41581881
PMCPMC12934295

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.