Evidence map›Paper›PMID 41581077›Full record

ArticleACS synthetic biology2026

The Coli Toolkit (CTK): An Extension of the Modular Yeast Toolkit for Use in

Jacob Mejlsted, Erik Kubaczka, Sebastian Wirth, Heinz Koeppl

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In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jacob MejlstedCentre for Synthetic Biology, TU Darmstadt, Darmstadt 64283, Germany.ORCID 0009-0006-4239-9023
Erik KubaczkaCentre for Synthetic Biology, TU Darmstadt, Darmstadt 64283, Germany.ORCID 0009-0005-2467-5533
Sebastian WirthCentre for Synthetic Biology, TU Darmstadt, Darmstadt 64283, Germany.
Heinz KoepplCentre for Synthetic Biology, TU Darmstadt, Darmstadt 64283, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic circuits are a cornerstone of synthetic biology, enabling programmable control of cellular behavior for applications in health, sustainability, and biotechnology. While Genetic Design Automation (GDA) tools have optimized and streamlined the design of such circuits, rapid and efficient assembly of DNA remains a bottleneck in the Design-Build-Test-Learn (DBTL) cycle. Here, we present the Coli Toolkit (CTK), a modular Golden Gate-based cloning system, adapted from the Yeast Toolkit (YTK) for use in

Indexed as

Escherichia coliSaccharomyces cerevisiaeSynthetic BiologyCloning, MolecularGene Regulatory NetworksGenetic EngineeringPromoter Regions, GeneticRibosomesRNA, CatalyticRNA, Catalyticgenetic design automationgolden gateMoClosynthetic biologytoolkit

Identifiers

PMID41581077
PMCPMC12930495

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.