Evidence map›Paper›PMID 41580881›Full record

ArticleEnvironmental microbiome2026

Exploring the diversity and potential of host-associated microorganisms in Hypnum leptothallum to enhance plant resilience to drought stress for karst desertification restoration.

Shasha Wu, Yihong Xiang, Xiaofan Chen, Yueyan Lu, Yubi Li, Zheyu He, Qianya Zuo, Wei Cao, Jiaojiao Qu

Abstract read
In one paragraph

Article in Environmental microbiome, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shasha WuKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Yihong XiangKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Xiaofan ChenKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Yueyan LuKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Yubi LiKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Zheyu HeKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Qianya ZuoKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China.
Wei CaoKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China. gdwcao@163.com.
Jiaojiao QuCollege of Tea Science, Guizhou University/Guizhou Key Laboratory of Agricultural Microbiology, Guiyang, 550025, China.

Funding

the National Natural Science Foundation of China 32060038the National Natural Science Foundation of China 32160026the Science and Technology Program of Guizhou Province QianKeHe-ZK[2022]034the Talent Fund of Guizhou University [2020]9
6 · The paper itself

Abstract

backgroundKarst rocky desertification poses a serious threat to the ecosystems of southwest China, where Hypnum leptothallum plays a crucial role in forming microbial crusts essential for restoration. However, the use of native microbial applications in this area remains largely unexplored.

resultsIn this study, the host-associated microbial communities of H. leptothallum from four severely desertified regions in Guizhou Province were characterized using high-throughput sequencing. The results revealed conserved α- and β-diversity, with dominant bacterial phyla being Pseudomonadota (34-47%) and Actinomycetota (23-35%), and fungal phyla being Ascomycota (57-83%) and Basidiomycota (14-32%). Subsequent carbon-source preference analysis guided the formulation of specialized media (e.g., α-D-lactose, N-acetyl-D-glucosamine) to isolate culturable strains, with cross-referencing identifying 14 bacterial and 36 fungal species consistently shared between sequencing and cultivation. Functional evaluation demonstrated bacterial dominance in inorganic phosphorus solubilization (68% of strains), protease synthesis (76%), ammonia production (56%), and indole-3-acetic acid biosynthesis (62%), while fungi excelled in organophosphorus solubilization. Further drought tolerance and gametophyte co-culture assays identified 10 drought-resistant bacterial strains and 16 strains significantly enhancing H. leptothallum growth within 7 days.

conclusionsThese functionally validated strains, particularly drought-adapted and growth-stimulating species closely related to Rhodococcus erythropolis, provide targeted microbial resources for developing synthetic inoculants to optimize artificial crust propagation in karst restoration.

Indexed as

Artificial community constructionArtificial moss crust cultivationHigh-throughput sequencingHypnum leptothallum-associated microorganismsKarst rocky desertification

Identifiers

PMID41580881
PMCPMC12911403

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.