ArticleParasites & vectors2026
An evaluation of longitudinal Anopheles stephensi egg viability and resistance to desiccation at different thermal conditions over time.
Article in Parasites & vectors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Anopheles stephensi: a new and important menace to South America?Revista da Sociedade Brasileira de Medicina Tropical · 2026Article
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6 authors.
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Abstract
purposeAnopheles stephensi, a malaria vector in South Asia and parts of the Middle East, has been detected as an invasive species in numerous African countries in recent years. It threatens to increase malaria disease burden and reverse gains made in malaria control and elimination. To halt further expansion, it is critical to understand the biological characteristics that may have facilitated An. stephensi range expansion. In its invasive range, An. stephensi larvae have been found to colonize artificial containers, many of which are shared with Aedes aegypti. The success of Ae. aegypti as an invasive vector is often attributed to the use of artificial containers and the ability of Ae. aegypti eggs to remain viable in the absence of water for months. While An. stephensi is found in artificial containers, it is unclear whether the eggs can remain viable without water for extended periods.
methodsIn this study, we used two laboratory strains of An. stephensi (SDA500 and STE2) and one Ae. aegypti strain (LVP-IB12) to evaluate 1) whether An. stephensi eggs can remain viable like Ae. aegypti when egg substrates are completely dried and 2) assess egg viability duration at varying temperatures when eggs are held on a moistened substrate in a high humidity environment.
resultsAn. stephensi egg viability and subsequent larval survival was observed consistently when moistened egg sheets were held at 15 ˚C in a high humidity environment for up to 14 days in both strains. An. stephensi eggs were not viable when completely dried, except when the protocol was amended to include a 15 °C storage temperature. Though egg viability and larval survival was observed in the amended protocol for SDA500 and STE2 (16% and 21% respectively), it was significantly less than that of LVP-IB12 (83%) and was only observed in the eggs stored for the shortest time point.
conclusionsThese findings suggest that An. stephensi may remain viable if eggs are transported under ideal conditions (15 ˚C and >75% RH) through trade routes. Thus, the persistence of An. stephensi eggs in the absence of water should be considered in programs that engage in surveillance and control of An. stephensi in Africa.
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