Evidence map›Paper›PMID 41579862›Full record

ArticleCell reports. Medicine2026

Pan-cancer N-glycoproteomic atlas of patient-derived xenografts uncovers FAT2 as an actionable surface target.

Meinusha Govindarajan, Salvador Mejia-Guerrero, Shawn C Chafe, Shahbaz Khan, Wei Shi, Matthew Waas, Martin A Rossotti, Amanda Khoo, Lydia Y Liu, Vladimir Ignatchenko and 34 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

44 authors.

Meinusha GovindarajanDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Salvador Mejia-GuerreroPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Shawn C ChafeCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada.
Shahbaz KhanPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Wei ShiPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Matthew WaasPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Martin A RossottiHuman Health Therapeutics Research Centre, Life Sciences Division, National Research Council Canada, Ottawa, ON K1A 0R6, Canada.
Amanda KhooDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Lydia Y LiuDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Vladimir IgnatchenkoPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Simona PrincipePrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Lusia SepiashviliDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Greg HussackHuman Health Therapeutics Research Centre, Life Sciences Division, National Research Council Canada, Ottawa, ON K1A 0R6, Canada.
Nazanin TatariCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
Chitra VenugopalCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada.
Petar MileticCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada.
Maxwell TopleyCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
Shan GrewalCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada.
Dillon McKennaCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
Lucas C AsselstineCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada.
Maria-Jose SandiPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Nhu-An PhamPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Alison CaseyPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Hyeyeon KimDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Christina KaramboulasPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Jalna MeensPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Peter BergqvistadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Begonia SilvaadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Patrick ChanadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Liza Cerna-PortilloadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Jasmine ChinadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Abilasha Rao-BhatiaadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Ming-Sound TsaoDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Rama KhokhaDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Susie SuDalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada.
Wei XuPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada; Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada.
David GoldsteinPrincess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada; Department of Otolaryngology-Head & Neck Surgery, University of Toronto, Toronto, ON M5S 3H2, Canada.
Laurie AillesDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Vuk StambolicDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada.
Fei-Fei LiuDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada; Department of Radiation Oncology, University of Toronto, Toronto, ON M5T 1P5, Canada.
Emma CumminsadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Ismael SamudioadMare BioInnovations, Vancouver, BC V6T 1Z3, Canada.
Sheila K SinghCentre for Discovery in Cancer Research, McMaster University, Hamilton, ON L8S 4M1, Canada; Department of Surgery, Faculty of Health Sciences, McMaster University, Hamilton, ON L8S 1C7, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada.
Thomas KislingerDepartment of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada; Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C1, Canada. Electronic address: thomas.kislinger@utoronto.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell surface proteins offer significant cancer therapeutic potential attributable to their accessible membrane localization and central roles in cellular signaling, yet their promise remains largely untapped due to technical challenges inherent to profiling them. Here, we employ N-glycoproteomics to analyze 85 patient-derived xenografts (PDXs), constructing Glyco PDXplorer-an in vivo pan-cancer atlas of cancer-derived surface proteins. We develop a target discovery pipeline to prioritize proteins with favorable expression profiles for immunotherapeutic targeting and validate FAT2 as a squamous-cancer-enriched surface protein minimally detected in normal tissue. Functional studies reveal that FAT2 is essential for head and neck squamous cancer (HNSC) cell growth and adhesion through regulation of surface architecture and integrin-PI3K signaling. Chimeric antigen receptor (CAR)-T cells targeting FAT2 demonstrate anti-tumor activity. This work lays the foundation for developing FAT2-targeted therapies and represents a pivotal platform to inform therapeutic target discovery across cancers.

Indexed as

CadherinsGlycoproteinsHead and Neck NeoplasmsProteomicsAnimalsCell Line, TumorHumansMiceSignal TransductionXenograft Model Antitumor AssaysCadherinsGlycoproteinsCAR-TFAT2head and neck squamous cancerimmunotherapyN-glycoproteomicspan-cancer atlaspatient-derived xenograftssquamous cell cancersurfaceometargeted therapy

Identifiers

PMID41579862
PMCPMC12923961

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.