Evidence map›Paper›PMID 41579593›Full record

ArticleStem cell research2026

Generation and characterization of POMC-tdTomato reporter human pluripotent stem cell lines.

Vukasin M Jovanovic, Rick Rausch, Maria Caterina DeRosa, David Castellano, Cody McKee, Chaitali Sen, Fiona Daly, Claudia A Doege, Carlos A Tristan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Vukasin M JovanovicNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States.
Rick RauschNaomi Berrie Diabetes Center, Columbia Stem Cell Initiative, Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, United States.
Maria Caterina DeRosaNaomi Berrie Diabetes Center, Columbia Stem Cell Initiative, Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, United States.
David CastellanoNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States.
Cody McKeeNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States.
Chaitali SenNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States.
Fiona DalyNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States.
Claudia A DoegeNaomi Berrie Diabetes Center, Columbia Stem Cell Initiative, Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, NY, United States. Electronic address: cad2114@cumc.columbia.edu.
Carlos A TristanNational Center for Advancing Translational Sciences, Division of Preclinical Innovation, National Institutes of Health, Rockville, MD, United States. Electronic address: carlos.tristan@nih.gov.

Funding

RESEARCH TRAININGP30DK026687 · NIDDK · ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS · PI Anthony W Ferrante, DYMPNA GALLAGHER · 1986 to 2026
$33.0M
Virtual systemic identification of drug targets of obesity candidate genesR01DK135938 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Yingchang Lu · 2023 to 2026
$2.9M
NIDDK NIH HHS P30 DK026687NIDDK NIH HHS R01 DK135938
6 · The paper itself

Abstract

Proopiomelanocortin (POMC) is a precursor polypeptide that undergoes extensive, tissue-specific post-translational processing. It is expressed in several tissues, including pituitary gland, hypothalamus, brain stem, and skin. The hypothalamic POMC neurons in the arcuate nucleus are major neuronal populations involved in the regulation of body weight. In these neurons, POMC is processed into several peptides, among them the anorexigenic alpha-melanocyte stimulating hormone. Thus, the POMC neurons in the ARC have been named "satiety" neurons and are highly desirable drug targets. Here, we performed CRISPR/Cas9-mediated insertion of tdTomato reporter at the endogenous POMC locus, enabling direct visualization of POMC expression through tdTomato fluorescence in human pluripotent stem cell (hPSC)-derived hypothalamic neurons. This reporter line enables real-time visualization of POMC neuron differentiation, and selective enrichment of these populations for molecular, functional, and pharmacological studies. This line is readily available as new alternative method (NAM) platform, to support disease modeling and drug discovery in metabolic and neuroendocrine disorders within a human context.

Indexed as

Genes, ReporterLuminescent ProteinsPluripotent Stem CellsPro-OpiomelanocortinCell DifferentiationCell LineHumansNeuronsRed Fluorescent ProteinLuminescent ProteinsPro-OpiomelanocortinRed Fluorescent ProteintdTomato

Identifiers

PMID41579593
PMCPMC12934563

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.