Purification and Comprehensive Characterization of Two Novel Acidic Glycoproteins from Scorpion with Therapeutic Potential.

Yusuf Ali, Naziermu Dongmulati, Zi Yang, Han He, Yanhua Gao, Haji Akber Aisa, Ahmidin Wali, Abulimiti Yili

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Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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8 authors.

Yusuf AliState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Naziermu DongmulatiState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Zi YangState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Han HeState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Yanhua GaoState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Haji Akber AisaState Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China.

Ahmidin Wali *State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China. ahmidin@ms.xjb.ac.cn.

Abulimiti Yili *State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, and the Key Laboratory of Chemistry of Plant Resources in Arid Regions Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40 - 1, Urumqi, China. abu@ms.xjb.ac.cn.

Funding

National Natural Science Foundation, Chinese Youth Foundation 82104017National Natural Science Foundation of China 22578472Xinjiang Tianchi Doctoral Project 2024000051Xinjiang Uygur Autonomous Region Tianshan Yingcai-Leading Talents in Scientific and Technological Innovation 2022TSYCLJ0008

6 · The paper itself

Abstract

Scorpions have long been utilized in traditional medicine for their therapeutic properties, including anti-cancer, analgesic, anti-thrombotic, and immune-modulating properties. This study aimed to optimize extraction conditions, partial purification, structural characterization and biological activity of glycoproteins from the scorpion (Buthus martensii Karsch) body. Under the optimized conditions, the extraction yield of scorpion glycoproteins (SGP's) reached 12.57 ± 0.7%. Subsequently, two novel glycoproteins, SGP-1a and SGP-1b, were isolated through DEAE-52 anion exchange chromatography and Sephadex G-75 gel filtration, with average molecular weights of 46.8 kDa and 10.3 kDa, respectively. Compositional analysis revealed SGP-1a and SGP-1b were rich in monosaccharides (Glu, Gal, Rha) and amino acids (Ser, Lys, Ala, Leu, Arg, Phe), and were characterized as homogeneous acidic protein-bound heteropolysaccharides. FTIR spectrum confirmed that SGP-1a and SGP-1b presence of glycated proteins, β-elimination reaction elucidated that SGP's contained N-glycosidic linkage. According to the CD analysis, SGP-1a contains 7.4% α-helix, 34.1% β-sheet, 13.2% β-turn, 34.6% random coil and SGP-1b contains 4.7% α-helix, 46.8% β-strand, 47.2% random coil. Bioactivity assays demonstrated significant anti-inflammatory activity against COX-2 (IC50: 21.14 ± 1.73 µg/mL for SGP-1a; 18.58 ± 1.54 µg/mL for SGP-1b) Meanwhile, both the SGPs exhibit strong antioxidant activity, the IC

Indexed as

GlycoproteinsScorpionsAnimalsAnti-Inflammatory AgentsAntioxidantsMiceAnti-Inflammatory AgentsAntioxidantsGlycoproteinsAntioxidant and Anti-Inflammatory activityGlycoproteinPurificationScorpion (Buthus Martensii karsch)Structural characterization

Identifiers

PMID41579258

DOI10.1016/j.meatsci.2013.09.004

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