ArticleFEMS microbiology ecology2026
The PceA-orthologous reductive dehalogenase of Dehalococcoides mccartyi CBDB1 is involved in 1,2,4-trichlorobenzene respiration.
Article in FEMS microbiology ecology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The reductive dehalogenation of halogenated benzenes by anaerobic bacteria is of great environmental and biotechnological importance; however, the role of specific reductive dehalogenases in the dehalogenation of different isomers has not been studied in detail. Here, we cultivated the obligate organohalide-respiring Dehalococcoides mccartyi strain CBDB1 with either 1,2,3- or 1,2,4-trichlorobenzene (TCB) as electron acceptor and investigated the transcription of its 32 reductive dehalogenase (rdhA) genes using RNA-sequencing. The chlorobenzene reductive dehalogenase gene cbrA, and rdhA cbdbA80 were the two most highly expressed rdhA genes with 1,2,3-TCB. In the presence of 1,2,4-TCB, cbrA was the most highly expressed rdhA followed by rdhA cbdbA1588, encoding an orthologue of the tetrachloroethene reductive dehalogenase PceA of D. mccartyi strain 195. RNA-sequencing also allowed for the detection of small RNAs and an unannotated protein. Proteomics confirmed the synthesis of RdhA CbdbA1588 during respiration with 1,2,4-TCB and also with hexachlorobenzene, which is dehalogenated via 1,2,4-TCB. Dehalogenase activity assays with cell extracts from 1,2,4-TCB-grown cultures indicated a higher activity towards 1,2,4-TCB and a ten-fold higher activity towards 2,3-dichlorophenol compared to that in extracts from 1,2,3-TCB-grown cultures. These findings demonstrate the functionality of RdhA CbdbA1588 and further support a role in 1,2,4-TCB dechlorination by strain CBDB1.
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