Evidence map›Paper›PMID 41578390›Full record

ArticleJournal of biological engineering2026

Preserving integrity: innovative in vitro methods for extracellular matrix decellularization and collagen purification.

Uliana Bashtanova, Rui Li, Ieva Goldberga, Sneha Bansode, Kathryn Gerl, Kristen Paige Burgess, Annika Janine Wegner-Repke, Melinda Jane Duer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Uliana BashtanovaYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Rui LiYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Ieva GoldbergaYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Sneha BansodeYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Kathryn GerlYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Kristen Paige BurgessYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Annika Janine Wegner-RepkeYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK.
Melinda Jane DuerYusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Rd, Cambridge, CB2 1EW, UK. mjd13@cam.ac.uk.

Funding

the European Union EXTREME 101019499
6 · The paper itself

Abstract

backgroundIn tissue engineering and cell therapy development, synthetic biomaterials are frequently supplemented with collagen or other extracellular matrix (ECM) components to enhance biocompatibility. To support these applications, novel methods for collagen purification and ECM decellularization were developed, with a focus on preserving the structural and biochemical integrity of the final products.

resultsThe effectiveness of these methods was validated using solid-state NMR and fluorescence spectroscopy, bright-field and confocal microscopy, amino acid analysis, proteomics and transmission electron microscopy. Intact cells were dislodged from ECM-producing cultures through the application of cytoskeleton-targeting drugs, while the native protein composition of the ECM was maintained. In parallel, collagen purified using chymotrypsin was shown to retain its native triple-helical structure and post-translational modifications.

conclusionsBoth techniques are broadly applicable to various cell types capable of producing collagen and/or ECM in vitro, thereby expanding the availability of species- and tissue-specific sources. These advances hold particular promise for human-relevant tissue engineering and drug discovery applications.

Indexed as

BiomaterialsCollagenCytoskeletonDecellularizationExtracellular matrixOrthovanadateTriple helixVinblastine

Identifiers

PMID41578390
PMCPMC12910738

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.