Evidence map›Paper›PMID 41578040›Full record

ArticleAnalytical and bioanalytical chemistry2026

Spatially resolved lipids in a mouse brain model of globoid cell leukodystrophy via IR-MALDESI MSI and parallel reaction monitoring MSI.

Sierra N Hunter, Mary F Wang, Brittany N Thomas, Anthony J Filiano, David C Muddiman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sierra N HunterBiological Imaging Laboratory for Disease and Exposure Research (BILDER), Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
Mary F WangBiological Imaging Laboratory for Disease and Exposure Research (BILDER), Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
Brittany N ThomasDepartment of Pathology, Duke University, Durham, NC, 27710, USA.
Anthony J FilianoMarcus Center for Cellular Cures, Duke University, Durham, NC, 27705, USA. anthony.filiano@duke.edu.
David C MuddimanBiological Imaging Laboratory for Disease and Exposure Research (BILDER), Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA. dcmuddim@ncsu.edu.

Funding

Development and Application of New Ionization Methods for Biological Mass SpectrometryR01GM087964 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI MUDDIMAN, DAVID C. · 2010 to 2025
$4.7M
NIGMS NIH HHS R01GM087964
6 · The paper itself

Abstract

Globoid cell leukodystrophy (GLD) is a genetic neurodegenerative disease caused by mutations in galactosylceramide β-galactosidase (GALC) that results in the accumulation of the cytotoxic sphingolipid, psychosine. As psychosine is a biomarker specific to GLD, identifying the most afflicted regions of the nervous system can assist in better understanding the disease mechanism. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) and parallel reaction monitoring were utilized to elucidate the spatial distribution of the psychosine analyte and confirm the identity of the ion in a sagittal section of a GALC-deficient mouse brain. The presence of the psychosine was increased in specific anatomical regions of the brain responsible for the bodily functions that are impaired by GLD (cerebellum and brain stem). Several electrospray solvent additives (dopants) have enhanced the detection of various analyte types but with little success in enhancing the detection of sphingolipids. This study investigates the usefulness of ammonium fluoride electrospray doping in the positive ion mode for lipidomic IR-MALDESI MSI analysis.

Indexed as

BrainBrain ChemistryLeukodystrophy, Globoid CellLipidsPsychosineSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnimalsDisease Models, AnimalMiceLipidsPsychosineGloboid cell leukodystrophyIR-MALDESIMass spectrometry imagingPsychosine

Identifiers

PMID41578040
PMCPMC12999646

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.