Dual recombinase-mediated endothelial cell-specific lineage tracing and ablation.

Jie Li, Mingjun Zhang, Xiuxiu Liu, Zhenqian Zhang, Mengyang Shi, Wenjuan Pu, Bin Zhou

Abstract read

In one paragraph

Article in Cell regeneration (London, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jie LiCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Mingjun ZhangCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Xiuxiu LiuCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Zhenqian ZhangCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Mengyang ShiCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Wenjuan PuCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. puwenjuan@sibcb.ac.cn.

Bin ZhouCAS CEMCS-CUHK Joint Laboratory, New Cornerstone Science Laboratory, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China. zhoubin@sibs.ac.cn.ORCID http://orcid.org/0000-0001-5278-5522

Funding

CAS Projcet for Young Scientists in Basic Research YSBR-012National Natural Science Foundation of China 32370783National Natural Science Foundation of China 32500629National Natural Science Foundation of China 82088101Shanghai Pilot Program for Basic Research-CAS, Shanghai Branch JCYJ-SHFY-2021-0the National Key Research & Development Program of China 2023YFA1800700the National Key Research & Development Program of China 2024YFA1803302

6 · The paper itself

Abstract

The precise ablation of specific cell lineages is crucial for functional studies in vivo. Conventional methods, like the Cre-dependent iDTR system, are constrained by the off-target effects and variable efficiency of single-recombinase approaches. Here, we present a novel Cdh5-RL-DTRGFP mouse model that requires both Dre and Cre recombinases to activate diphtheria toxin receptor (DTR) and GFP expression specifically in endothelial cells. This dual-recombinase logic ensures tight control over transgene expression. We demonstrate that diphtheria toxin administration in recombined mice leads to efficient endothelial cell ablation, resulting in severe vascular leakage, rapid organ failure, and mortality. The Cdh5-RL-DTRGFP line thus provides a robust and precise platform for the genetic dissection of endothelial cell function in physiological and pathological contexts.

Indexed as

Cdh5Cell ablationCre-loxPDre-roxDual recombinasesEndothelial cells

Identifiers

PMID41577894

DOI10.1186/s13619-026-00280-2

PMCPMC12830524

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.