Evidence map›Paper›PMID 41577702›Full record

ArticleNature communications2026

An H5N1 clade 2.3.4.4b virus vaccine that elicits cross-protective antibodies against conserved domains of H5 and N1 glycoproteins.

Eduard Puente-Massaguer, Thales Galdino Andrade, Michael J Scherm, Kirill Vasilev, Hassanein Abozeid, Alesandra J Rodriguez, Joshua Yueh, Disha Bhavsar, John D Campbell, Dong Yu and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Eduard Puente-MassaguerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0002-2816-7051
Thales Galdino AndradeDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.
Michael J SchermDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Kirill VasilevDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Hassanein AbozeidDepartment of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0002-5433-6939
Alesandra J RodriguezDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.
Joshua YuehDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Disha BhavsarDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.ORCID http://orcid.org/0000-0003-1564-3298
John D CampbellDynavax Technologies Corporation, Emeryville, CA, USA.ORCID http://orcid.org/0000-0002-8526-3330
Dong YuDynavax Technologies Corporation, Emeryville, CA, USA.
Richard J WebbyDepartment of Host Microbe Interactions, St Jude Children's Research Hospital, Memphis, TN, USA.ORCID http://orcid.org/0000-0002-4397-7132
Yoshihiro KawaokaDepartment of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.ORCID http://orcid.org/0000-0001-5061-8296
Gabriele NeumannDepartment of Pathobiological Sciences, Influenza Research Institute, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA.
Julianna HanDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-3402-2609
Andrew B WardDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.ORCID http://orcid.org/0000-0001-7153-3769
Florian KrammerDepartment of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. florian.krammer@mssm.edu.ORCID http://orcid.org/0000-0003-4121-776X

Funding

COLLABORATIVE INFLUENZA VACCINE INNOVATION CENTER: UNIVERSAL INFLUENZA VACCINE RESEARCH75N93019C00051 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KRAMMER, FLORIAN · 2019 to 2025
$105.4M
New York City Virus Hunters - A Community Science InitiativeR25GM150146 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Florian Krammer, Christine Marizzi · 2023 to 2026
$1.1M
NIAID NIH HHS 75N93019C00051NIGMS NIH HHS R25 GM150146
6 · The paper itself

Abstract

The continuous evolution and global spread of highly pathogenic avian influenza (HPAI) H5N1 viruses, particularly clade 2.3.4.4b, pose major challenges for pandemic preparedness. This study evaluates a low-dose inactivated split-virus vaccine derived from H5N1 clade 2.3.4.4b, formulated with an Alum/CpG adjuvant, in a preclinical female mouse model. The vaccine induces strong humoral and cellular immunity, generating high titers of cross-reactive antibodies against diverse H5 hemagglutinin (HA) and across different N1 neuraminidase (NA) glycoproteins. The Alum/CpG adjuvant supports substantial antigen dose sparing and promotes a balanced Th1/Th2 profile. Functional assays show potent virus neutralization, neuraminidase inhibition, and antibody-dependent cellular cytotoxicity, alongside robust antigen-specific CD4

Indexed as

Antibodies, ViralCross ProtectionHemagglutinin Glycoproteins, Influenza VirusInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesNeuraminidaseOrthomyxoviridae InfectionsViral ProteinsAnimalsAntibodies, NeutralizingCross ReactionsFemaleHumansMiceMice, Inbred BALB CAntibodies, NeutralizingAntibodies, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza VaccinesNA protein, influenza A virusNeuraminidaseViral Proteins

Identifiers

PMID41577702
PMCPMC12913899

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.