Evidence map›Paper›PMID 41576045›Full record

ArticlePLoS biology2026

Overexpression of the pioneer transcription factor Nr5a2 promotes the development of mouse somatic cell nuclear transfer embryos.

Yanhua Zhao, Meiting Zhang, Yan You, Jinrong Zhang, Yuning Ji, Lei Lei

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yanhua ZhaoDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Meiting ZhangDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Yan YouDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Jinrong ZhangDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Yuning JiDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Lei LeiDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0002-5239-7009

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Somatic cell nuclear transfer (SCNT) is a valuable tool in regenerative medicine, yet its efficiency remains limited by epigenetic reprogramming barriers that have been partially corrected by global regulation of epigenetic enzymes. However, these approaches lack gene locus specificity and may disrupt normal gene regulation. Therefore, new strategies capable of broadly enhancing reprogramming fidelity are needed. Here, we demonstrate that overexpression of the pioneer transcription factor Nr5a2 in mouse SCNT embryos improves both zygotic genome activation and the morula-to-blastocyst transition, two major developmental barriers in SCNT, and enhances birth rates. Mechanistically, Nr5a2 recruits P300 to increase H3K27ac at genes with low expression, restoring transcriptional activity and promoting SCNT embryo development.

Indexed as

Embryonic DevelopmentNuclear Transfer TechniquesReceptors, Cytoplasmic and NuclearAnimalsBlastocystEmbryo, MammalianEpigenesis, GeneticFemaleGene Expression Regulation, DevelopmentalHistonesMiceMorulaZygoteHistonesNr5a2 protein, mouseReceptors, Cytoplasmic and Nuclear

Identifiers

PMID41576045
PMCPMC12829815

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.