Evidence map›Paper›PMID 41576012›Full record

ArticlePloS one2026

High-throughput single-fly LC-MS/MS for quantitative profiling of biogenic amines in Drosophila.

Aki Hori, Takahiro Nitta, Kei-Ichiro Inamori, Hirotaka Kanoh, Takayuki Kuraishi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Aki HoriFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.
Takahiro NittaDivision of Glycopathology, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
Kei-Ichiro InamoriDivision of Glycopathology, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Sendai, Miyagi, Japan.
Hirotaka KanohInstitute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, Japan.
Takayuki KuraishiFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa, Japan.ORCID https://orcid.org/0000-0002-9493-6082

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present an LC-MS/MS method that enables reproducible, quantitative detection of multiple biogenic amines-including octopamine, dopamine, serotonin, and their precursors-from single Drosophila individuals. The workflow involves only a minimal extraction in methanol prior to direct injection, eliminating the need for derivatization or laborious purification steps. Its simplicity ensures high reproducibility and broad applicability across experimental settings. Pilot applications revealed distinct neurochemical responses across biologically relevant conditions: a circadian rise in octopamine at dusk, acute increases in octopamine, dopamine, and serotonin under cold stress, and elevations of octopamine, serotonin, and tryptophan during starvation. These findings highlight the utility of single-head LC-MS/MS for dissecting neuromodulator dynamics at the level of individual organisms.

Indexed as

Biogenic AminesDrosophilaDrosophila melanogasterLiquid Chromatography-Mass SpectrometryTandem Mass SpectrometryAnimalsChromatography, LiquidDopamineHigh-Throughput Screening AssaysOctopamineReproducibility of ResultsSerotoninBiogenic AminesDopamineOctopamineSerotonin

Identifiers

PMID41576012
PMCPMC12829853

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.