ArticleFrontiers in cellular and infection microbiology2025
Rapid and visual detection of Tacheng tick virus 1 using loop-mediated isothermal amplification technique.
Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Tacheng tick virus 1 (TcTV-1) is an emerging tick-borne nairovirus associated with human febrile illness. To date, no reliable detection method for TcTV-1 has been established. In this study, we developed and evaluated a rapid loop-mediated isothermal amplification (LAMP) assay for the detection of TcTV-1. Methods: The primers were designed based on the nucleocapsid protein (NP) gene of TcTV-1. Sensitivity was assessed using ten-fold serial dilutions of recombinant plasmids containing the target sequence. Specificity was evaluated using cDNA from Songling virus (SGLV), Yezo virus (YEZV), Tick-borne encephalitis virus (TBEV), Severe fever with thrombocytopenia syndrome virus (SFTSV), and Beiji nairovirus (BJNV). The assay was validated using field-collected tick samples. Results: The TcTV-1-specific LAMP assay detected as few as 1×10 Discussion: We report a TcTV-1-specific LAMP assay with high sensitivity, specificity, and cost-effectiveness, making it a practical tool for use in field-based or resource-limited settings.
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