RECC: A Red/ET-CRISPR/Cas9-based system enabling genome mining of marine

Zong-Jie Wang, Haibo Zhou, Youming Zhang, Fu Yan, Liujie Huo, Xiaotong Wang

Abstract read

In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

6 authors.

Zong-Jie WangHelmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

Haibo ZhouHelmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

Youming ZhangHelmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

Fu YanHunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha, 410081, China.

Liujie HuoHelmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

Xiaotong WangHelmholtz International Laboratory, State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marine microorganisms possess vast biosynthetic potential, yet most of their biosynthetic gene clusters (BGCs) remain transcriptionally silent under laboratory conditions. Genetic intractability has been a major barrier to activating these cryptic pathways. Here, we present RECC, an integrated Red/ET-CRISPR/Cas9 system that enables seamless, marker-free genome editing in marine bacteria. RECC couples Red/ET recombineering with CRISPR/Cas9-mediated cleavage, allowing the incorporation of homology arms and protospacers into a single construct through one-step Gibson assembly, thereby substantially simplifying the engineering process. Using

Indexed as

Genome miningNatural productsPseudoalteromonasRecombinases

Identifiers

PMID41574265

DOI10.1016/j.synbio.2025.12.015

PMCPMC12819058

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.