Evidence map›Paper›PMID 41573878›Full record

ArticlebioRxiv : the preprint server for biology2025

Reprogramming the Rossmann Fold Signature Motif Creates Orthogonal Redox Biocatalysts.

Yu Ping, Jin Young Kim, Minh-Anh L Dinh, Emma Luu, Youtian Cui, Edward King, William B Black, Justin B Siegel, Han Li

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yu PingDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697, USA.
Jin Young KimDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697, USA.
Minh-Anh L DinhDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697, USA.
Emma LuuGenome Center, University of California, Davis, Davis, CA 95616, USA.
Youtian CuiGenome Center, University of California, Davis, Davis, CA 95616, USA.
Edward KingDepartment of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA.
William B BlackDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697, USA.
Justin B SiegelGenome Center, University of California, Davis, Davis, CA 95616, USA.
Han LiDepartment of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697, USA.

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI YOUNG, THOMAS MICHAEL · 1987 to 2021
$50.1M
Enzymatic formation of the hydrocarbon skeletons underlying diterpenoid diversityR01GM076324 · NIGMS · IOWA STATE UNIVERSITY · PI PETERS, REUBEN JOHN · 2006 to 2019
$3.4M
Expanding the Metabolic Currencies of LifeR35GM153401 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Han Li · 2024 to 2026
$1.4M
NIEHS NIH HHS P42 ES004699NIGMS NIH HHS R01 GM076324NIGMS NIH HHS R35 GM153401
6 · The paper itself

Abstract

Biological reducing power is carried by nicotinamide adenine dinucleotide (phosphate) (NAD(P)/H), which supports cellular functions and cannot be specifically directed to engineered metabolic pathways. Nicotinamide mononucleotide (NMN(H)) has emerged as an orthogonal redox cofactor to address this challenge, yet strategies remain elusive for creating NMN(H)-specific enzymes that no longer interact with the cellular NAD(P)/H pools. Previous designs avoided perturbing the most ancient and conserved GxGxxG motif in Rossmann fold enzymes, the root cause of persistent NAD(P)/H recognition. Herein, we demonstrated that this motif, though long considered essential, is in fact mutable to yield active NMN

Identifiers

PMID41573878
PMCPMC12822633

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.