Evidence map›Paper›PMID 41573572›Full record

ArticleFrontiers in immunology2025

Nuclear cell-free DNA on the loose: an early warning signal of ischemia-reperfusion injury in kidney transplantation.

Gabriel Strandberg, Rebecca Trattner, Myriam Martin, Carl M Öberg, Carl Raihle, Oleg Slivca, Shahnawaz Alam, Mårten Segelmark, Anders Christensson, Bo Nilsson and 3 more

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Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Gabriel Strandberg *Department of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.
Rebecca Trattner *Department of Translational Medicine, Lund University, Malmö, Sweden.
Myriam MartinDepartment of Translational Medicine, Lund University, Malmö, Sweden.
Carl M ÖbergDepartment of Clinical Sciences Lund, Faculty of Medicine, Lund University, Lund, Sweden.
Carl RaihleDepartment of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.
Oleg SlivcaDepartment of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.
Shahnawaz AlamDepartment of Pathology and Laboratory Medicine, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
Mårten SegelmarkDepartment of Clinical Sciences Lund, Faculty of Medicine, Lund University, Lund, Sweden.
Anders ChristenssonDepartment of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.
Bo NilssonDepartment of Immunology, Genetics and Pathology (IGP), Rudbeck Laboratory C5:3, Uppsala University, Uppsala, Sweden.
Clara PaulDepartment of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.
Anna M BlomDepartment of Translational Medicine, Lund University, Malmö, Sweden.
Ali-Reza BiglarniaDepartment of Clinical Sciences Malmö, Faculty of Medicine, Lund University, Malmö, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Cell-free DNA is an emerging marker of allograft injury, yet its role in the immediate phase of ischemia-reperfusion injury remains incompletely understood. Methods: In this prospective cohort of 127 kidney transplant recipients (86 deceased donors, 41 living donors), intraoperative plasma samples were collected systemically pre-implantation and from the allograft vein postreperfusion. Nuclear and mitochondrial cell-free DNA were quantified, alongside subset assessments of neutrophil extracellular trap markers and soluble C5b-9 as a marker of thromboinflammation. An in vitro necrosis model of human proximal tubular cells evaluated the concordance between cell injury and C5b-9 generation. Results: An immediate and sustained release of nuclear, but not mitochondrial, cell-free DNA was observed post-reperfusion, predominantly in deceased donor kidneys. This release corresponded with cold ischemic time and delayed graft function while showing temporal correlations with soluble C5b-9, indicating that cell-free DNA release parallels thromboinflammatory activation upon reperfusion. Conclusion: Immediate nuclear cellfree DNA release upon reperfusion reflects intragraft injury linked to downstream thromboinflammatory activation, underscoring the impact of early ischemia-reperfusion injury.

Indexed as

Cell-Free Nucleic AcidsCell NucleusKidney TransplantationReperfusion InjuryAdultBiomarkersComplement Membrane Attack ComplexExtracellular TrapsFemaleHumansMaleMiddle AgedProspective StudiesBiomarkersCell-Free Nucleic AcidsComplement Membrane Attack Complexcell-free DNAcell injuryischemia-reperfusion injurykidney transplantationthromboinflammation

Identifiers

PMID41573572
PMCPMC12819266

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