ArticleBioengineering & translational medicine2026
Vitrification and rapid rewarming of precision-cut liver slices for pharmacological and biomedical research.
Article in Bioengineering & translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Biobanked Liver Organoids: A Roadmap for Precision Hepatology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Vitrification and rapid rewarming of precision-cut liver slices for pharmacological and biomedical research.Bioengineering & translational medicine · 2026Article
- Precision-cut tumor slices: a biomimetic tool for accelerating oncological development from basic research to clinical translation.Frontiers in physiology · 2026Review
- An Immunological and Translational Framework for Interspecies Exogenic Liver Transplantation.Cell transplantationReview
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13 authors.
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Abstract
High-throughput in vitro pharmacological toxicity testing is essential for drug discovery. Precision-cut liver slices (PCLS) provide a robust system for screening that is representative of the complex 3D structure of the whole liver. However, PCLS are not available as off-the-shelf products. Cryopreservation could solve this bottleneck by effectively preserving PCLS indefinitely until their time of use. With cryopreservation, slices could be shipped to laboratories without access to fresh tissue or used in planned experiments independent of surgical schedules. Here, we explore an "ice-free" cryopreservation approach called vitrification and focus on culturing and assessing PCLS for 3 days post-vitrification and rewarming, given that most acute drug toxicity tests are conducted over 24 h. Rat liver slices were diffusively loaded with a cryoprotective agent (CPA) cocktail consisting of ethylene glycol and sucrose. The CPA-loaded PCLS were placed on a polymer cryomesh, vitrified in liquid nitrogen, and rapidly rewarmed in CPA. The vitrified and rewarmed PCLS were subsequently cultured in serum-free media for 3 days. The cryopreserved PCLS maintained high viability, morphology, function, enzymatic activity, and drug toxicity response. Results show that the vitrified PCLS perform comparably to untreated controls and significantly outperform conventionally cryopreserved PCLS in all assessments (
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Registered trials
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