ArticleBMC genomics2026
Enhanced soybean salt tolerance and oil content via marker-assisted pyramiding of GmSALT3 and high-oil QTLs.
Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
objectiveSoil salinity and demand for edible oil are two major challenges facing global soybean production. Traditional breeding approaches often encounter negative correlations between salinity tolerance and seed-quality traits. This study aimed to overcome this trade-off by pyramiding the salt tolerance gene GmSALT3 with multiple oil content quantitative trait loci (QTL) through marker-assisted selection.
methodsUsing a cross between the salt-tolerant line, 2125, and high-oil cultivar Neiminda 3, we developed backcross (BC₃F₅) populations and employed kompetitive allele-specific polymerase chain reaction genotyping for five markers linked to GmSALT3 and three major oil QTL (qOil-5-1, qOil-10-1, qOil-15). A total of 892 BC₃F₂ plants were screened, with 3.8% carrying the complete pyramided genotype.
resultsPhenotypic evaluation revealed successful improvement in both traits: pyramided lines showed a 42.3% reduction in salt injury index under 150-mM NaCl hydroponic stress (F₅,₂₁₀ = 87.3, P < 0.001) and a 2.8% increase in seed oil content compared with the recurrent parent (t₆₈ = 6.84, P < 0.001). Multi-environment trials across two locations (Nanjing: 32°03’N, 118°46’E, pH: 6.8, electrical conductivity [EC]: 1.3 dS/m; Yancheng: 33°23’N, 120°08’E, pH: 7.2, EC: 2.8 dS/m) over 2 years demonstrated stable performance with no yield penalty under non-saline conditions. Trade-off analysis indicated modest positive synergy between traits (r = 0.31, P < 0.05, n = 70), suggesting the potential to mitigate expected negative correlations. Best linear unbiased prediction analysis confirmed genotype stability with minimal genotype–environment interaction (17.5% of total variation, indicating robust performance).
conclusionThis study demonstrates the feasibility of simultaneously improving stress tolerance and quality traits through strategic gene pyramiding, although the relatively low recovery frequency (3.8%) of fully pyramided genotypes highlights technical challenges. The pyramided lines provide valuable germplasm for sustainable soybean production in marginal lands, acknowledging limitations in QTL coverage and the need for broader validation across additional environments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.