Evidence map›Paper›PMID 41571921›Full record

ArticleWorld journal of microbiology & biotechnology2026

A modular single- and dual-gene expression toolkit for Kluyveromyces marxianus.

Zewei Lu, Changhui Lv, Zhuoer Chen, Zhiwei Zhu, Xiaoqiang Ma

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Zewei Lu *Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Changhui Lv *Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Zhuoer ChenDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Zhiwei ZhuMOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
Xiaoqiang MaDepartment of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. xq_ma@sjtu.edu.cn.

Funding

Food Safety Risk Assessment Project from the National Health Commission of the PRC Specialty Laboratory of Food Safety Risk Assessment and Standard Development RA-2024-2, RA-2025-2.1General Project of Natural Science Foundation of Shanghai, Shanghai Municipal Science and Technology Commission 25ZR1401186National Key Research and Development Program of China 2022YFD2101304National Natural Science Foundation of China 22177018Shanghai Collaborative Innovation Center of Agri-Seeds ZXWH3150101/002Start-up fund of Shanghai Jiao Tong University WH220415004
6 · The paper itself

Abstract

Kluyveromyces marxianus is a fast-growing, food-grade yeast with broad substrate utilization, however, its limited expression tools hinder its synthetic biology applications. Here, we developed single- and dual-gene expression systems based on screened elements, including a promoter pENO and a bidirectional promoter pHTX. These systems enabled stable expression across multiple K. marxianus strains, with expression levels affected by genetic element combinations, carbon source, and culture time. Using the single-gene system with a short intergenic sequence (IGG) for bicistronic expression, the production of leghemoglobin from Vigna angularis (VaHB) reached 30.4 mg/L using galactose as carbon source. In contrast, the dual-gene system achieved 48.4 mg/L of VaHB with the co-expression of HEM1, enhancing heme biosynthesis using glucose as a carbon source. Additionally, Simian Virus 40 (SV40) nuclear localization signals (NLS) directed fluorescent proteins to the nucleus, enabling subcellular targeting. This toolkit supports efficient and context-responsive gene expression in K. marxianus, facilitating its development as a versatile microbial chassis for industrial protein production and synthetic biology applications.

Indexed as

Gene ExpressionKluyveromycesFungal ProteinsGalactoseGene Expression Regulation, FungalGlucoseNuclear Localization SignalsPromoter Regions, GeneticFungal ProteinsGalactoseGlucoseNuclear Localization SignalsExpression systemsKluyveromyces marxianusNuclear localizationProtein expression

Identifiers

PMID41571921

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.