Evidence map›Paper›PMID 41571281›Full record

ArticleNano letters2026

Dual-Color Expansion Microscopy of Membrane Proteins Using Bioorthogonal Labeling.

Steven Edwards, Birthe Meineke, Sebastian Bauer, Hans Blom, Simon Elsässer, Hjalmar Brismar

Abstract read
In one paragraph

Article in Nano letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Steven EdwardsScience for Life Laboratory, KTH Royal Institute of Technology, 171 21 Solna, Sweden.
Birthe MeinekeScience for Life Laboratory, Karolinska Institute, 171 21 Solna, Sweden.
Sebastian BauerScience for Life Laboratory, Karolinska Institute, 171 21 Solna, Sweden.
Hans BlomScience for Life Laboratory, KTH Royal Institute of Technology, 171 21 Solna, Sweden.
Simon ElsässerScience for Life Laboratory, Karolinska Institute, 171 21 Solna, Sweden.ORCID 0000-0001-8724-4849
Hjalmar BrismarScience for Life Laboratory, KTH Royal Institute of Technology, 171 21 Solna, Sweden.ORCID 0000-0003-0578-4003

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With recent advances in fluorescence microscopy, resolution is often limited by the size of the label and the resulting linkage error, rather than the microscope itself. Site-specific incorporation of noncanonical amino acids (ncAAs) combined with bioorthogonal click chemistry provides a powerful tool for fluorescent protein labeling, overcoming the spatial uncertainty inherent to antibody-based probes. Here, we present a method to further improve labeling precision by combining ncAA labeling with expansion microscopy (ExM) for dual-color super-resolution imaging. After optimizing labeling procedures and fluorophore selection, we visualize and resolve the nanoscale distribution of Na,K-ATPase α

Indexed as

Fluorescent DyesMembrane ProteinsAmino AcidsClick ChemistryHEK293 CellsHumansMicroscopy, FluorescenceStaining and LabelingAmino AcidsFluorescent DyesMembrane ProteinsBioorthogonal chemistryExpansion microscopyLinkage errorNoncanonical amino acidsSite-specific labelingSuper-resolution microscopy

Identifiers

PMID41571281
PMCPMC12879918

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.