ArticleCell reports methods2026
High-efficiency transfection of Acanthamoeba castellanii using a cationic polymer.
Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Article
- Development of Approaches for Transgene Expression in the Pathogenic Free-Living AmoebaPathogens (Basel, Switzerland) · 2025Article
- Recombinant protein expression inFrontiers in bioengineering and biotechnology · 2025Article
- Optical imaging and gene transfection potential of linear polyethylenimine-coated AgTurkish journal of biology = Turk biyoloji dergisi · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The free-living amoeba Acanthamoeba castellanii is an ecologically, clinically, and evolutionarily important microorganism. A. castellanii amoebae can be directly pathogenic to humans and serve as reservoirs for bacterial pathogens (e.g., Legionella pneumophila), but they also regulate the proliferation of other microorganisms in the soil. Despite their importance, no reliable genetic system has been developed, hampering the use of A. castellanii and related species as model organisms. Transfecting A. castellanii with plasmids is possible with commercial kits, but it is expensive, inefficient, and vulnerable to product discontinuation. In this contribution, we present a method for efficient transfection of A. castellanii with readily available and inexpensive polyethylenimines. We systematically explore the method's parameters, achieving up to 100-fold higher efficiency compared to currently used protocols. This method provides a robust step toward a complete genetic toolbox for A. castellanii, thereby expanding its utility as a model organism.
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Registered trials
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