Acute hepatic injury and metabolic response in hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) to high-dose Vibrio harveyi infection.

Zhuojin He, Xiaomin Zheng, Lixin Ma, Xing Lei, Zhilong Chen, Li Lin, Fei Shi

Abstract read

In one paragraph

Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Zhuojin He *Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Xiaomin Zheng *Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Lixin MaGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Xing LeiGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Zhilong ChenGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Li LinGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.

Fei ShiGuangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China. fshi1203@126.com.

Funding

Provincial organization and implementation project of provincial rural revitalization strategy in 2024, and Demonstration and promotion of high-quality and efficient whole-industry chain technology focusing on fishing industry in rural areas NJTG20240255The National Natural Science Foundation of China 4210061024

6 · The paper itself

Abstract

The hybrid groupers (Epinephelus fuscoguttatus female × Epinephelus lanceolatus male) have been seriously harmed by Vibrio harveyi, but the acute damage and the underlying immune defense mechanisms during infection are still unknown. In this study, fish in the treatment group were challenged with 200 µL of V. harveyi suspension (1.01 × 10⁸ CFU/mL) to investigate the mechanisms underlying V. harveyi infection in hybrid grouper by integrating histopathology, biochemistry, and high-resolution transcriptomics. Histopathological analyses revealed that V. harveyi infection induced severe liver apoptosis, as evidenced by TdT-mediated dUTP nick-end labeling assays. Biochemical assays indicated a significant increase in glutamic-pyruvic transaminase (GPT; P < 0.05), while malondialdehyde (MDA) was significantly decreased (P < 0.05). Moreover, transcriptomic profiling revealed significant enrichment in the p53 signaling pathway, alanine, aspartate and glutamate metabolism, and cholesterol metabolism. Quantitative real-time polymerase chain reaction further confirmed the significant upregulation of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and Toll-like receptor 3 (TLR-3). In addition, redundancy analysis revealed the correlations between enzyme activities and differentially expressed genes. Overall, these results demonstrate that V. harveyi infection compromises hepatic health in hybrid grouper by inducing structural damage to the liver, perturbing oxidative stress, and impairing both immune function and metabolic processes.

Indexed as

Fish DiseasesPerciformesVibrioVibrio InfectionsAnimalsAquacultureChimeraFemaleMaleApoptosisHepatic healthHybrid grouperTranscriptomicsVibrio harveyi

Identifiers

PMID41569350

DOI10.3390/genes16070839

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