ArticleFrontiers in microbiology2025
Development and validation of a novel triplex droplet digital PCR assay for simultaneous detection of African swine fever virus, pseudorabies virus, and porcine parvovirus.
Article in Frontiers in microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Development and Validation of an RT-Droplet Digital PCR Assay for Detection of Peste des Petits Ruminants.Veterinary sciences · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coinfection with multiple viruses significantly complicates swine disease management. DNA viruses including African swine fever virus (ASFV), pseudorabies virus (PRV), and porcine parvovirus (PPV) are causing reproductive failure in sows, with overlapping clinical presentations that challenge differential diagnosis. To address this, we developed a novel triplex droplet digital PCR (ddPCR) assay using three distinct fluorescent probe sets for simultaneous detection of these three viruses. The multiplex ddPCR demonstrated superior analytical performance with high specificity, sensitivity, and reproducibility. When compared to conventional quantitative PCR (qPCR), the ddPCR assay achieved limits of detection (LOD) of 0.08, 3.41, and 3.38 copies/μL for ASFV, PRV, and PPV respectively-representing approximately 10-fold lower LOD values than corresponding qPCR methods. Cross-reactivity tests confirmed absolute specificity against other important swine pathogens. Clinical validation using 217 field samples revealed marked diagnostic advantages: ddPCR detected positive cases in 137 samples (63.1%) versus 115 (53.0%) by qPCR. Species-specific positivity rates showed ddPCR improved detection by 5.99% (ASFV), 0.46% (PRV), and 3.69% (PPV) compared to qPCR. Concordance analysis demonstrated strong agreement between methods (94.01-99.54%), with ddPCR showing superior sensitivity particularly for low-viral-load samples. This study establishes the first multiplex digital PCR platform for simultaneous differential diagnosis of ASFV, PRV, and PPV. The assay provides a robust tool for enhanced surveillance and control of these economically significant pathogens in swine populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.