ArticleCellular and molecular life sciences : CMLS2026
Hypoxic mesenchymal stem cell-Derived Exosomal circPTP4A2 improves granulosa cell mitochondrial function via YBX1.
Article in Cellular and molecular life sciences : CMLS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Immediate protective effect of rUCMSC-EVs on ovarian function in a cyclophosphamide -induced premature ovarian insufficiency rats: counteracting granulosa cell apoptosis.Journal of ovarian research · 2026Article
- Isorhamnetin-preconditioned MSC-derived exosomes restore ovarian function by inhibiting ferroptosis in chemotherapy-induced POF.Stem cell research & therapy · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundPremature ovarian insufficiency (POI) profoundly compromises female reproductive health through accelerated follicle depletion and endocrine disruption. Emerging evidence highlights the therapeutic potential of mesenchymal stem cell-derived exosomes (MSC-Exs), particularly when their function is enhanced by hypoxic preconditioning. In this study, the ability of hypoxia-preconditioned MSC-Exs (H-Exs) to ameliorate oxidative damage to granulosa cells (GCs) and restore ovarian function, was systematically evaluated, and a POI rat model was used to investigate the underlying mechanism.
methodsCircRNAs specifically expressed in H-Exs were identified and validated. The ability of H-Exs and their corresponding circPTP4A2 to repair oxidative damage and restore mitochondrial function were evaluated by antioxidant enzyme assays, reactive oxygen species (ROS) assays, JC-1 staining, ATP level assays, oxygen consumption rate (OCR) measurements and TEM. The interaction between circPTP4A2 and YBX1 was analysed by molecular dynamics simulations, RIP, CHX assays, and MG132 assays, and the restorative effect of the circPTP4A2/YBX1 axis on ovarian function was verified.
resultsOur findings revealed that compared with normoxic MSC-Exs (N-Exs), H-Exs exerted superior protective effects, significantly attenuating oxidative stress and restoring mitochondrial bioenergetics in KGN cells. Mechanistically, circPTP4A2 was identified as a hypoxia-responsive cargo selectively enriched in H-Exs. This circular RNA stabilized Y-box binding protein 1 (YBX1) through direct interaction, increasing its antioxidative capacity and mitochondrial regulatory functions. Hypoxia-inducible factor 1-alpha (HIF-1α) was further shown to transcriptionally upregulate circPTP4A2 via direct binding to the promoter region of its host gene PTP4A2.
conclusionThese results establish the circPTP4A2/YBX1 axis as a critical mediator of the therapeutic efficacy of H-Exs for POI, providing both mechanistic insights and a translational framework for exosome-based regenerative strategies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.