Evidence map›Paper›PMID 41565816›Full record

ArticleNature2026

Construction of complex and diverse DNA sequences using DNA three-way junctions.

Noah Evan Robinson, Weilin Zhang, Rajesh Ghosh, Bryan Gerber, Hanqiao Zhang, Charles Sanfiorenzo, Sixiang Wang, Dino Di Carlo, Kaihang Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Noah Evan RobinsonDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Weilin ZhangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Rajesh GhoshBioengineering Department, University of California, Los Angeles, CA, USA.ORCID 0000-0002-7408-8944
Bryan GerberDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Hanqiao ZhangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Charles SanfiorenzoDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.
Sixiang WangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA.ORCID 0009-0007-0441-1930
Dino Di CarloBioengineering Department, University of California, Los Angeles, CA, USA.ORCID 0000-0003-3942-4284
Kaihang WangDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. kaihangwang@caltech.edu.ORCID 0000-0001-7657-8755

Funding

Megabase Scale Genome Engineering and Synthesis in Mammalian CellsDP2GM140937 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI WANG, KAIHANG · 2020 to 2020
$2.4M
NIGMS NIH HHS DP2 GM140937NSF Division of Biological Infrastructure grant
6 · The paper itself

Abstract

The ability to construct entirely new synthetic DNA sequences de novo is essential to engineering and studying biology. However, the ability to produce long complex synthetic DNA sequences and libraries currently lags behind the ability to sequence and edit DNA

Indexed as

DNAGenetic EngineeringSynthetic BiologyBase CompositionBase SequenceGene LibraryDNA

Identifiers

PMID41565816
PMCPMC12979194

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.