Evidence map›Paper›PMID 41565654›Full record

ArticleNature communications2026

Phospholipid composition strongly affects the assembly of β barrel proteins into purified bacterial outer membranes.

Thushani D Nilaweera, Nathan T Brandes, Ian S LaCroix, Benjamin Schwarz, Harris D Bernstein

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Dark Side ofJournal of the American Chemical Society · 2026
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Thushani D NilaweeraGenetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.ORCID 0009-0007-1057-4998
Nathan T Brandes *Proteins and Chemistry Section, Research and Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.ORCID 0009-0001-6958-9285
Ian S LaCroix *Proteins and Chemistry Section, Research and Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.ORCID 0000-0002-4859-7024
Benjamin SchwarzProteins and Chemistry Section, Research and Technologies Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.ORCID 0000-0002-5894-953X
Harris D BernsteinGenetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. harris_bernstein@nih.gov.ORCID 0000-0002-4941-3741

Funding

Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) Intramural researchU.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) Intramural research
6 · The paper itself

Abstract

Virtually all integral outer membrane proteins (OMPs) produced by Gram-negative bacteria contain a unique 'β barrel' structure that serves as a membrane spanning domain. The universal barrel assembly machine (BAM) catalyzes OMP assembly (folding and membrane insertion) in vivo, and purified Escherichia coli BAM that is reconstituted into proteoliposomes catalyzes OMP assembly in vitro. Here we show that BAM also catalyzes the assembly of OMPs into outer membrane fractions ('native OMs') that are purified by optimized conventional methods. Interestingly, we found that OMP assembly was moderately impaired when native OMs were isolated from a mlaA

Indexed as

Bacterial Outer MembraneBacterial Outer Membrane ProteinsEscherichia coliEscherichia coli ProteinsPhospholipidsMutationPhospholipid Transfer ProteinsBacterial Outer Membrane ProteinsBamA protein, E coliEscherichia coli ProteinsMlaA protein, E coliPhospholipidsPhospholipid Transfer Proteins

Identifiers

PMID41565654
PMCPMC12923597

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.