Evidence map›Paper›PMID 41563528›Full record

ArticleMolecular biology reports2026

Molecular cloning, characterization, and expression analysis of two GABA

Shu-Fan Yu, John Praygod Kimaro, Xu Zhou, Shuang Li, Li-Bing Zheng, Chang-Feng Chi

Abstract read
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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Shu-Fan Yu *National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China.
John Praygod Kimaro *National and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China.
Xu ZhouNational and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China.
Shuang LiNational and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China.
Li-Bing ZhengNational and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China. zhenglb@bbc.edu.cn.
Chang-Feng ChiNational and Provincial Joint Engineering Research Centre for Marine Germplasm Resources Exploration and Utilization, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Changzhi Island, Lincheng, Zhoushan, 316022, China. chichangfeng@hotmail.com.

Funding

National Natural Science Foundation of China 32473136National Natural Science Foundation of China 42406102Natural Science Foundation of Zhejiang Province, China LTGN24C190005
6 · The paper itself

Abstract

backgroundGABAA receptor-associated proteins (GABARAPs) are evolutionarily conserved ubiquitin-like modulators known for their roles in γ-aminobutyric acid (GABA) receptor trafficking and autophagy. METHODS AND

resultsIn this research, two GABARAPs’ full-length cDNA sequences from the common Chinese cuttlefish Sepiella japonica (GABAA receptor-associated protein and GABAA receptor-associated protein like 2, SjGABARAP and SjGABARAPL2) were cloned and analyzed, with accession numbers PQ423738 and PQ423739, respectively. Both genes encode 117-amino-acid proteins, each containing a conserved ubiquitin-like domain typical of the autophagy-related protein 8 (ATG8) family. Sequence alignments and phylogenetic analysis confirmed that both proteins are highly conserved across invertebrates and vertebrates, with significant homology to GABARAP and GABARAPL2 from other species. Subcellular localization studies demonstrated that SjGABARAP and SjGABARAPL2 are primarily localized in the cytoplasm, and part of SjGABARAPL2 is also found in the nucleus. In situ hybridization revealed that both SjGABARAP and SjGABARAPL2 significant signals were notably observed in brain, optic lobe, and white body, with additional signals in pancreas of SjGABARAPL2. Furthermore, quantitative real-time PCR (qRT-PCR) analysis showed temporal and tissue-specific expression patterns, with high levels of SjGABARAPL2 in the pancreas, liver, gill, intestine, brain, and optic lobes.

conclusionsThese findings are expected to lay a basis for the next exploration of cell signaling and immune regulation of GABARAPs in S. japonica.

Indexed as

Adaptor Proteins, Signal TransducingMicrotubule-Associated ProteinsAmino Acid SequenceAnimalsCloning, MolecularPhylogenyReceptors, GABA-ASequence AlignmentAdaptor Proteins, Signal TransducingMicrotubule-Associated ProteinsReceptors, GABA-ACephalopodCloningExpressionGABARAPGABARAPL2Sepiella japonica

Identifiers

PMID41563528

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.