Evidence map›Paper›PMID 41563282›Full record

ArticleJournal of applied oral science : revista FOB2026

Biofilm formation of mixed Candida albicans and methicillin-sensitive Staphylococcus aureus and surface properties of a 3D-printed denture base resin under different printing parameters.

Larianne S Moisés, Hamile Emanuella do Carmo Viotto, Sabrina Romão Gonçalves Coelho, Danny Omar Mendoza Marin, Raphael Freitas Souza, Ana Carolina Pero

Abstract readEvaluation Study
In one paragraph

Article in Journal of applied oral science : revista FOB, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Larianne S MoisésUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, SP, Brasil.
Hamile Emanuella do Carmo ViottoUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, SP, Brasil.ORCID http://orcid.org/0000-0001-5725-6101
Sabrina Romão Gonçalves CoelhoUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, SP, Brasil.
Danny Omar Mendoza MarinUniversidade Federal de Santa Catarina, Centro de Ciências da Saúde, Departamento de Odontologia, Florianópolis, SC, Brasil.
Raphael Freitas SouzaUniversité Laval, Faculty of Dental Medicine, Quebec, Canada.ORCID http://orcid.org/0000-0001-8437-9991
Ana Carolina PeroUniversidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araraquara, Departamento de Materiais Dentários e Prótese, Araraquara, SP, Brasil.ORCID http://orcid.org/0000-0002-0203-2386

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the formation of mixed-species biofilms of Candida albicans and methicillin-sensitive Staphylococcus aureus (MSSA) on the surface of a 3D-printed denture base resin, as well as its surface properties, under varying printing parameters. METHODOLOGY: Discs (n=40 per group, 10×1.2 mm) of a denture base resin (priZma 3D Bio Denture) were fabricated using two 3D-printers-Liquid Crystal Display (LCD) and Digital Light Processing (DLP)-at three different angles (0°, 45°, or 90°). Surface roughness was measured using a digital profilometer and expressed as Ra (µm). For surface energy (SE) analysis, contact angles were measured using a tensiometer. Discs were incubated at 37 °C for 90 minutes and 48 hours to enable biofilm formation using C. albicans and MSSA inocula. Cell viability was assessed by colony-forming unit (CFU/mL) counts, and metabolic activity was evaluated using the XTT assay (absorbance). Microbial counts and XTT results were analyzed by three-way ANOVA (printer type, printing angle, incubation period). Surface roughness was analyzed by two-way ANOVA (printer type, printing angle), with Tukey's test and a significance level of 0.05.

resultsFor both CFU/mL and XTT assays, incubation period was the only significant factor (p<0.001 and p=0.006, respectively), while other factors and interactions were not statistically significant (p>0.05). Surface roughness was significantly influenced by printer type, printing angle, and their interaction (p=0.027). The LCD 0° and LCD 90° groups produced smoother surfaces compared with LCD 45° (p=0.002), which showed similar values to all DLP groups regardless of angle (p>0.05). The DLP printer did not show significant roughness variations across the tested angles (p>0.05). The LCD groups presented numerically lower SE values compared to the DLP groups.

conclusionThe LCD system performs better than DLP in reducing surface roughness at 0° and 90°. Moreover, the analyzed factors did not significantly affect microbial adhesion or the formation of mixed-species biofilms.

Indexed as

Acrylic ResinsBiofilmsCandida albicansDenture BasesPrinting, Three-DimensionalStaphylococcus aureusAnalysis of VarianceColony Count, MicrobialMaterials TestingMicroscopy, Electron, ScanningReference ValuesReproducibility of ResultsSurface PropertiesTime FactorsAcrylic Resins

Identifiers

PMID41563282
PMCPMC13123784

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.