ArticleAnalytical chemistry2026
Mid-Infrared Spectroscopy for the Qualitative and Quantitative Analysis of the Wheat Proteome.
Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Wheat contributes 19% of protein in the global human diet. Mid-infrared spectroscopy (MIR) combined with attenuated total reflection (ATR) is an excellent analytical method for assessing the chemical composition of complex biological samples. In contrast to established techniques used for wheat protein analysis, we show that by using ATR-MIR the protein secondary structures of different fractions can be studied. We found that albumin and globulin fractions were primarily composed of α-helix, with proportions of 57.8% and 45.9%, respectively. Gliadins, meanwhile, contained 38.3% β-turn and 36.9% α-helix, while glutenins predominantly exhibited 44.8% β-turn secondary structures. In addition, we found that by using analysis of variance (ANOVA) simultaneous component analysis (ASCA), the obtained MIR spectra of the wheat protein fractions were significantly (
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