Evidence map›Paper›PMID 41562311›Full record

ArticleCell biology international2026

Engineering Immortalized Bovine Granulosa Cells Using a Triple-Gene Approach: Mutant CDK4, Cyclin D1, and TERT.

Lanlan Bai, Minami Takahashi, Jin Kobayashi, Takahiro Eitsuka, Himari Matsusaka, Taku Ozaki, Eriko Sugano, Hiroshi Tomita, Yuan Xu, Kazuhiro Kawamura and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell biology international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Lanlan BaiDepartment of Life Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
Minami TakahashiSchool of Food Industrial Sciences, Miyagi University, Sendai, Miyagi, Japan.
Jin KobayashiSchool of Food Industrial Sciences, Miyagi University, Sendai, Miyagi, Japan.ORCID https://orcid.org/0000-0001-8267-1315
Takahiro EitsukaGraduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan.
Himari MatsusakaGraduate School of Science and Engineering, Iwate University, Morioka, Iwate, Japan.
Taku OzakiDepartment of Life Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
Eriko SuganoDepartment of Life Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
Hiroshi TomitaDepartment of Life Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
Yuan XuDepartment of Obstetrics and Gynaecology, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo, Japan.
Kazuhiro KawamuraDepartment of Obstetrics and Gynaecology, Juntendo University Faculty of Medicine, Bunkyo-ku, Tokyo, Japan.
Kiyotaka NakagawaGraduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Japan.
Tohru KiyonoExploratory Oncology Research & Clinical Trial Center, National Cancer Center, kashiwanoha, Chiba, Japan.
Tomokazu FukudaDepartment of Life Sciences, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advancing reproductive technologies in livestock is essential to improve both productivity and genetic potential of cattle. Despite this importance, application of reproductive biotechnologies in cattle breeding remains limited. Bovine granulosa cells (bGCs), which are key components of the ovarian follicle, are critical in female reproduction as they produce steroid hormones and growth factors necessary for oocyte development. However, primary bGCs exhibit restricted proliferative capacity in vitro, limiting their utility in large-scale studies on mechanisms related to follicular development. To address this limitation, we attempted to immortalize bGCs by co-expressing human mutant cyclin-dependent kinase 4 (CDK4

Indexed as

Cyclin D1Cyclin-Dependent Kinase 4Granulosa CellsTelomeraseAnimalsAromataseCattleCell CycleCell ProliferationFemaleHumansMutationAromataseCyclin D1Cyclin-Dependent Kinase 4TelomeraseTERT protein, humanaromatasecattlegranulosa cellsimmortalizationK4DT

Identifiers

PMID41562311
PMCPMC12821082

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.