ArticleBMC plant biology2026
Comparative transcriptomic and transcript-based network analyses revealed genotype-specific alternative splicing induced by Sclerotinia sclerotiorum in Brassica napus.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundSclerotinia sclerotiorum is a destructive necrotrophic fungus, that causes stem rot in Brassica napus, severely reducing yield worldwide. While host resistance is shaped by complex transcriptional and post-transcriptional regulation, the contribution of alternative splicing (AS) to cultivar-specific resistance in B. napus remains poorly understood.
resultsWe conducted an integrative transcriptomic analysis of three resistant and three susceptible B. napus cultivars pre- and post-inoculation to characterize genotype-specific AS, gene expression changes, and transcript-based co-expression networks during pathogen infection. A total of 1,176 differentially alternatively spliced (DAS) genes were identified from the comparison between the transcriptomes of the infection-induced (II) and cultivar-related (CR) groups. Surprisingly, we found that 91% of DAS genes were genotype specific, highlighting the strong cultivar dependence of AS responses. Intron retention was the predominant AS event, and ~ 80% of the DAS genes were also differentially expressed, suggesting a complex connection between splicing and expression regulation. Weighted gene co-expression network analysis (WGCNA) based on transcripts identified key modules related to the pathogen response, identifying hub regulators involved in membrane trafficking, transcriptional control, and stress-associated metabolism. Prominent DAS genes such as SEC14-like lipid transfer protein (SFH8), FKBP17, and transcription factors such as HCA2, VAL2, and WRI4 could strongly implicated in immune signaling and hormonal pathways.
conclusionOur findings establish AS as a critical and genotype-dependent regulatory layer in B. napus defense against S. sclerotiorum. Linking splicing dynamics with co-expression networks and highlighting key hub regulators can pave the way for improving B. napus resistance in the future.
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