Evidence map›Paper›PMID 41558085›Full record

ArticleJournal of inorganic biochemistry2026

Phosphoester hydrolysis using a de novo designed protein: A combined experimental and computational study.

Dona Imanga Upamadi Edirisinghe, Prerana Dash, Jiawen Yang, Sreerag N Moorkkannur, Deepa Neupane, Jithma Gethmini Piyasara Sriwardana Dissanayake, Alexis Eckhart, Katlyn Meier, Rajeev Prabhakar, Olga V Makhlynets

Abstract read
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In one paragraph

Article in Journal of inorganic biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dona Imanga Upamadi EdirisingheDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, United States of America.
Prerana DashDepartment of Chemistry and Biochemistry, Baylor University, One Bear Place, Waco, TX 76798, United States of America.
Jiawen YangDepartment of Chemistry, University of Miami, Coral Gables, FL 33146, United States of America.
Sreerag N MoorkkannurDepartment of Chemistry, University of Miami, Coral Gables, FL 33146, United States of America.
Deepa NeupaneDepartment of Chemistry, University of Miami, Coral Gables, FL 33146, United States of America.
Jithma Gethmini Piyasara Sriwardana DissanayakeDepartment of Chemistry and Biochemistry, Baylor University, One Bear Place, Waco, TX 76798, United States of America.
Alexis EckhartDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, United States of America.
Katlyn MeierDepartment of Chemistry, University of Miami, Coral Gables, FL 33146, United States of America. Electronic address: kmeier@miami.edu.
Rajeev PrabhakarDepartment of Chemistry, University of Miami, Coral Gables, FL 33146, United States of America. Electronic address: rpr@miami.edu.
Olga V MakhlynetsDepartment of Chemistry and Biochemistry, Baylor University, One Bear Place, Waco, TX 76798, United States of America. Electronic address: olga_makhlynets@baylor.edu.

Funding

Metalloproteins: evolution, catalysis and material designR35GM155013 · NIGMS · BAYLOR UNIVERSITY · PI OLGA MAKHLYNETS · 2024 to 2026
$933k
From Fundamental Studies of Metalloproteins to Practical ApplicationsR15GM148939 · NIGMS · SYRACUSE UNIVERSITY · PI MAKHLYNETS, OLGA · 2022 to 2022
$310k
NIGMS NIH HHS R15 GM148939NIGMS NIH HHS R35 GM155013
6 · The paper itself

Abstract

Metallohydrolases use metal cations in their active sites to catalyze the hydrolytic cleavage of biological substrates. We used a simple model protein Due Ferri single chain (DFsc) in combination with Mn-Mn and Zn-Zn to study the effect of the nature of metal ions on its reactivity. The ICP-OES analysis confirmed the metal ion binding with desired ratios. The Mn

Indexed as

ProteinsHydrolysisManganeseModels, MolecularZincManganeseProteinsZincDe novo designDinuclear centerEPRMechanismMetal bindingPhosphoester hydrolysis

Identifiers

PMID41558085
PMCPMC13472179

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.