Simultaneous Determination of Glyphosate, Aminomethylphosphonic Acid, and Glufosinate in Green Coffee Beans by LC-MS/MS: Optimization, Validation, and Field Study.

Júlio César R M da Silva, Millena Christie F Avelar, Márcia C M Ribeiro, Mariana de O Almeida, Vanessa H F de Faria, Vanessa M Osório, Adriana F Faria

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Article in Journal of agricultural and food chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Júlio César R M da SilvaDepartment of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.

Millena Christie F AvelarPesticides Residues Laboratory - Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil.

Márcia C M RibeiroPesticides Residues Laboratory - Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil.

Mariana de O AlmeidaPesticides Residues Laboratory - Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil.

Vanessa H F de FariaPesticides Residues Laboratory - Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil.

Vanessa M OsórioDepartment of Chemistry and Physics - Center of Exact, Natural and Health Sciences, Universidade Federal do Espírito Santo, 29500-000 Alegre, ES, Brazil.

Adriana F FariaDepartment of Chemistry, Institute of Exact Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.ORCID 0000-0002-7320-4907

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite the widespread use of glyphosate in coffee crops, there is a lack of simple, low-cost, validated methods for detecting glyphosate and related compounds in green coffee beans, the primary export form subject to international regulation. This study optimized and validated a liquid chromatography with tandem mass spectrometry method for the determination of glyphosate, aminomethylphosphonic acid (AMPA), and glufosinate in green coffee, using a modified QuPPe-PO extraction followed by a cleanup combining liquid-liquid extraction and dispersive solid-phase extraction. Validation followed SANTE/11312/2021 guidelines, confirming the method's linearity, precision, accuracy, and quantification limits. Limits of quantification were 0.02 mg kg

Indexed as

AminobutyratesCoffeaGlycineHerbicidesLiquid Chromatography-Mass SpectrometryOrganophosphonatesTandem Mass SpectrometryChromatography, High Pressure LiquidFood ContaminationGlyphosateIsoxazolesOrganophosphorus CompoundsSeedsSolid Phase Extraction1-aminomethylphosphonic acidAminobutyratesaminomethylphosphonic acid (AMPA)GlycineGlyphosateHerbicidesIsoxazolesOrganophosphonatesOrganophosphorus Compoundsphosphinothricindispersive solid-phase extractiongreen coffeepolar herbicidesQuPPe-POvalidation

Identifiers

PMID41556766

DOI10.1021/acs.jafc.5c09979

PMCPMC12879917

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