Effect of high hydrostatic pressure on D-amino acid oxidase activity and stability.

Marisabel Morales-Muralles, Spencer J Serrano, George Makhatadze, José I Reyes-De-Corcuera

Abstract read

In one paragraph

Article in Journal of biological engineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marisabel Morales-MurallesDepartment of Agricultural and Biological Engineering, IFAS University of Florida, Gainesville, FL, 32611, USA.ORCID http://orcid.org/0009-0008-4732-5431

Spencer J SerranoDepartment of Agricultural and Biological Engineering, IFAS University of Florida, Gainesville, FL, 32611, USA.ORCID http://orcid.org/0000-0002-9416-0317

George MakhatadzeRensselaer Polytechnic Institute, Troy, NY, 12180, USA.ORCID http://orcid.org/0000-0003-4565-1264

José I Reyes-De-CorcueraDepartment of Agricultural and Biological Engineering, IFAS University of Florida, Gainesville, FL, 32611, USA. jireyes@ufl.edu.ORCID http://orcid.org/0000-0001-6275-6899

Funding

NSF Research Coordination Network MCB 1817845

6 · The paper itself

Abstract

We report the effects of high hydrostatic pressure (HHP) on D-amino acid oxidase (DAAO) from pork kidney stability and activity. At 20 °C, the activity decreased with increasing pressure, but at 40 °C, it was not significantly affected because the increased catalytic rate compensated for the enzyme inactivation within the time frame of the experiment. At 50-300 MPa, raising the temperature increased the rate of DAAO inactivation, indicating that increases in both pressure and temperature contributed to inactivation. In contrast to other oxidases pressure did not stabilize DAAO against thermal inactivation. Treatments between 0.1 and 100 MPa had no significant effect on its stability at 55 °C. However, the rate of inactivation increased with increasing pressure from 100 to 300 MPa, resulting in an activation volume (ΔV

Indexed as

Catalytic rateD-amino acid oxidaseEnzyme stabilizationHigh hydrostatic pressureInternal protein cavitiesThermal inactivation

Identifiers

PMID41555337

DOI10.1186/s13036-025-00604-x

PMCPMC12964626

What OpenQuestion holds

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.