Evidence map›Paper›PMID 41555025›Full record

ArticleNature nanotechnology2026

Spatiotemporal targeting of messenger RNA lipid nanoparticles to the endometrium for the treatment of reproductive disorders.

Saed Abbasi, Jairo Ortiz, Kimberly Bockley, Hongyu Feng, Emily Chen, Jordan Miller, Marina Better, Charles Eberhart, Neomi Jerry, Justin Hanes and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. RNA therapeutics: current status and future directions.Signal transduction and targeted therapy · 2026
    Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Saed AbbasiCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA. sabbasi5@jhmi.edu.ORCID http://orcid.org/0000-0001-8874-233X
Jairo OrtizCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Kimberly BockleyCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-2072-0326
Hongyu FengCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Emily ChenCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jordan MillerCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0001-7641-3061
Marina BetterCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Charles EberhartDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Neomi JerryCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Justin HanesCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0001-9672-9821
James H SegarsDepartment of Gynecology and Obstetrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Laura M EnsignCenter for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA. lensign@jhmi.edu.ORCID http://orcid.org/0000-0002-6949-7911

Funding

Nanomedicine approaches for prevention of inflammation-induced preterm birthR01HD103124 · NICHD · JOHNS HOPKINS UNIVERSITY · PI BURD, IRINA, ENSIGN, LAURA · 2021 to 2025
$3.3M
Role of senescent cells in uterine fibroid pathogenesisR01HD111243 · NICHD · JOHNS HOPKINS UNIVERSITY · PI Mostafa A. Borahay, JENNIFER H ELISSEEFF · 2023 to 2026
$3.2M
Nanomedicine-based approach for characterizing the epigenome in prevention of inflammation-induced preterm birth.R01HD108905 · NICHD · JOHNS HOPKINS UNIVERSITY · PI IRINA BURD, Laura Ensign · 2023 to 2026
$2.4M
NICHD NIH HHS R01 HD103124NICHD NIH HHS R01 HD108905NICHD NIH HHS R01 HD111243U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) R01HD108905
6 · The paper itself

Abstract

Dysfunctions of the endometrium, the uterus inner lining, can impair embryo implantation and reduce pregnancy rates. Intrauterine administration of cytokines has shown potential to improve endometrium function, but it is challenged by poor targeting and dose-limiting systemic side effects. Here we present a strategy for introducing therapeutic messenger RNA into the endometrium for the treatment of reproductive disorders. mRNA was loaded into a ligand-conjugated lipid nanoparticle (LNP), enabling multivalent interactions with the temporally overexpressed integrin receptors on the endometrial surface during the window of implantation. Conjugating the targeting ligand directly to the lipid component enhanced endometrial protein expression after intrauterine infusion and reduced systemic expression in the liver and spleen. A single infusion of granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA-loaded LNP sustained local protein expression for several hours and reduced GM-CSF systemic exposure. In a murine model of endometrial injury, GM-CSF mRNA-loaded LNP improved embryo implantation rates, outperforming recombinant GM-CSF. Our strategy demonstrates the efficacy of using mRNA to improve fertility outcomes.

Indexed as

EndometriumGranulocyte-Macrophage Colony-Stimulating FactorLipidsNanoparticlesRNA, MessengerAnimalsEmbryo ImplantationFemaleHumansLiposomesMicePregnancyGranulocyte-Macrophage Colony-Stimulating FactorLipid NanoparticlesLipidsLiposomesRNA, Messenger

Identifiers

PMID41555025
PMCPMC13017501

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.