Evidence map›Paper›PMID 41554713›Full record

ArticleBone research2026

Thrombospondin 1 and 2 regulate mesenchymal progenitor cell fate and matrix organization.

Madysen K Hunter, Sneha Korlakunta, Neda Vishlaghi, Monisha Mittal, Kyle Cragg, Conan Juan, Chase A Pagani, Yuxiao Sun, Lindsey Lammlin, Karen Kessell and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bone research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. An integrated investigation of systemic and localized pathophysiological mechanisms of stroke and multimodal therapeutic strategies.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Madysen K HunterCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.
Sneha KorlakuntaCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0009-0008-7336-6579
Neda VishlaghiCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.
Monisha MittalCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0009-0004-7773-4106
Kyle CraggDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.
Conan JuanCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0000-0002-0202-4298
Chase A PaganiCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0000-0001-9834-2516
Yuxiao SunCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.
Lindsey LammlinDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.
Karen KessellDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.
Dylan FeistCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.
Ji Hae ChoiCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0009-0001-1408-3383
Meng-Lun HsiehCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.
Jahnu SaikiaDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
Craig L DuvallDepartment of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.ORCID 0000-0003-3979-0620
Heeseog KangCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0000-0002-2612-8585
Andrea I AlfordDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.
Kurt D HankensonDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.ORCID 0000-0001-6361-143X
Robert J TowerCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA.ORCID 0000-0001-5856-5758
Tristan MaerzDepartment of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA.
Benjamin LeviCenter for Organogenesis, Regeneration and Trauma, University of Texas Southwestern, Dallas, TX, USA. Benjamin.Levi@utsouthwestern.edu.

Funding

Impacts of mechanosensation and matrix architecture on cell fate specification in traumatic heterotopic ossification - diversity supplementR01AR078324 · NIAMS · UT SOUTHWESTERN MEDICAL CENTER · PI LEVI, BENJAMIN · 2021 to 2025
$2.4M
NIAMS NIH HHS R01 AR078324U.S. Department of Defense (United States Department of Defense) HT9425-23-1-0327
6 · The paper itself

Abstract

Thrombospondin 1 and 2 (TSP1 and TSP2) are critical regulators of extracellular matrix (ECM) interactions, influencing cell differentiation and tissue repair. Recent discoveries from our laboratory and others highlight the importance of altered ECM alignment in influencing aberrant mesenchymal progenitor cell (MPC) differentiation and subsequent ectopic bone formation in trauma-induced heterotopic ossification (HO). However, the key regulators of this MPC to ECM interaction have yet to be elucidated. This study uncovers the role of matricellular TSP1 and TSP2 in MPC/ECM interaction as well as HO formation and progression. Using single-cell RNA sequencing, spatial transcriptomics, and in vivo models, we found that TSP1 is upregulated in tissue remodeling macrophages and MPCs at the injury site, while TSP2 is restricted to MPCs surrounding the HO anlagen. TSP1/2 double knockout (DKO) mice exhibited significantly reduced HO volume and disrupted ECM alignment. These findings highlight the crucial roles of TSP1 and TSP2 in musculoskeletal injury repair as well as HO formation and progression, supporting the potential to therapeutically target TSP1 and TSP2 to prevent HO.

Indexed as

Extracellular MatrixMesenchymal Stem CellsThrombospondin 1ThrombospondinsAnimalsCell DifferentiationMiceMice, KnockoutOssification, HeterotopicThrombospondin 2Thbs1 protein, mouseThrombospondin 1Thrombospondin 2Thrombospondins

Identifiers

PMID41554713
PMCPMC12816047

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.